摘要
目的探讨柴胡+丹参+五味子(CHAIDANWU)对肝硬化大鼠的作用及其机制。方法复制大鼠CCl4肝硬化模型,给予CHAIDANWU醇提物治疗,同时体外以醇提物和含药血清作用正常与模型大鼠肝细胞。测定血清酶水平、血清与匀浆HYP、PCI、PCIII含量,TNF-α、CD14i、NOS、MMP-1、TIMP-1在肝组织内阳性表达率,TUNEL法检测肝组织细胞凋亡,DNA琼脂糖凝胶电泳检测体外培养肝细胞凋亡,采用标准积分对肝细胞的变性及胶原纤维增生程度进行评价。结果CHAIDANWU能明显地降低血清酶活性,体外试验与体内结果一致。血清和肝组织中PCI、PCIII含量,肝组织TNF-α、CD14i、NOS、MMP-1、TIMP-1阳性率及肝细胞凋亡率明显降低,阻滞肝细胞的变性及胶原纤维增生。结论CHAIDANWU阻滞CCl4诱导大鼠肝硬化的作用,这种作用可能与抑制肝非实质激活,细胞因子释放减少,贮脂细胞释放TIMP-1壁降低,胶原纤维分解加速,肝细胞凋亡数减少有关。
Objective To investigate the effects and mechanism of CHAIDANWU compatibility on liver cirrhosis rat. Method： prepared the model of rat with liver cirrhosis induced by CCl4, then the CHAIDANWU was administrered by ig intragastric alcohol extract of the drug and serum containing drug were co-cultured with hepatic cell of rat in normal control and model in vitro. It was detected that the level of serum enzyme,and the contents of procollagen Ⅰ（PCI）,procollagen Ⅲ（PCI） and hydroxyproline（HYP）, and the expression positive rate of TNF-α, iNOS, MMp-1 ,TIMP-1 in hepatic tissue of rat cirrhosis in treated and untreated with CHAIDANWU .The apoptosis of hepatic cell in liver tissue was detected by TUNEL and agarose gel electrophoresis. The degree of liver cell degeneration necrosis and proliferation collagen fibrin were appraised by criterion integral. Result：CHAIDANWU decreased the level of serum enzyme,the contents of PC Ⅰ,PCⅢ ,in serum and liver homogenate,and the expression of TNF-α,CD14,iNOS,MMP-1 ,TIMP-1 in liver tissue,and blocked the degeneration necrosis of hepatic cell and proliferation of collagen fibrin. Conclussion：CHAIDANWU inhibits of rat cirrhosis induced by CCl4, which Correlates with depressing the activity of kupffer cell, reducing the cytokine release and TIMP-1 release by HSC ,and lowering hepatic cell apoptosis.
出处
《胃肠病学和肝病学杂志》
CAS
2005年第6期610-614,共5页
Chinese Journal of Gastroenterology and Hepatology
