摘要
[目的]采用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)鉴别一定比例掺伪的特种乳中所含的牛乳成分及确定最低检测掺伪比例。[方法]按一定的比例(1∶1、1∶4、1∶10、1∶20、1∶100、1∶200和1∶400(V牛乳/V特种乳))掺伪,离心分离乳清蛋白,SDS-PAGE电泳,确定掺伪差异性蛋白并进行灰度分析。[结果]SDS-PAGE可以快速、有效地分离各乳乳清蛋白以及掺伪的牛乳乳清蛋白。[结论]SDS-PAGE鉴别掺伪的最低比例为1∶10(V_(牛乳_/V_(特种乳))。
[Objective] The aim was to use SDS-PAGE to detect adulterated non-bovine milk and measure bovine milk content and confirm the lowest adulteration ratio.[Method]By using different ratio of（1∶1, 1∶4, 1∶10, 1∶20, 1∶100, 1∶200 and 1∶400（Vbovine milk/Vnon-bo-vine milk） ）adulterated non-bovine milk, whey protein was isolated and SDS-PAGE was conducted to locate the indicating protein then use gray analysis to detect adulteration and calculate bovine milk content.[Result] The SDS-PAGE can efficiently separate each animal’s whey protein as well as bovine whey protein from adulterated whey.[Conclusion] The lowest adulterating ratio can be detected with SDS-PAGE as 1∶10 （Vbovine milk/Vnon-bovine milk）.
出处
《安徽农业科学》
CAS
2016年第23期99-102,共4页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目"基于特种乳差异蛋白组分析建立乳间掺伪检测方法研究"(31471645)
