摘要
目的:考察玛咖粉对肾阳虚证卵巢早衰(POF)模型大鼠卵巢的养护作用。方法:将40只成年性成熟大鼠(♀)随机分为正常组、模型组、淫羊藿组[阳性药物,1 g/(kg·d)]和玛咖粉低、高剂量组[2.5、5 g/(kg·d)],每组8只。除正常组外,其余各组大鼠每天im氢化可的松[32 mg/(kg·d)],连续12 d,复制肾阳虚证POF模型。成模后,各给药组大鼠ig相应药物,正常组和模型组大鼠ig纯净水,每天上、下午各给药1次,连续14 d。观察各组大鼠体质量、摄食量、卵巢指数、子宫指数、动情周期以及血清生殖激素[雌二醇(E2)、卵泡刺激素(FSH)、促黄体生成素(LH)]水平的变化。结果:给药结束后,与正常组比较,模型组大鼠体质量、卵巢指数降低,动情周期紊乱,血清中E2含量减少,血清中FSH、LH水平以及FSH/LH比值升高(P<0.05或P<0.01)。与模型组比较,淫羊藿组和玛卡粉高剂量组大鼠动情周期紊乱情况、卵巢指数以及血清生殖激素水平得到明显改善(P<0.05);玛卡粉低剂量组大鼠仅卵巢指数和血清中LH水平明显改善(P<0.05),其余各指标差异均无统计学意义(P>0.05)。结论:高剂量玛咖粉能较好地恢复肾阳虚证POF大鼠的卵巢功能,恢复紊乱的动情周期,改善卵巢指数和血清生殖激素水平,对卵巢有一定的养护作用。
OBJECTIVE:To investigate the maintenance effects of Lepidium meyenii powder on ovary of premature ovarian failure(POF)model rats with Kidney-Yang deficiency pattern.METHODS:40 sexually mature rats(9)were randomly divided to normal group,model group,epimedium group[positive drug,1 g/(kg,d)],L.meyenii powder low-dose and high-dose groups[2.5,5 g/(kg.d)]with 8 rats in each group.Except for normal group,those groups were given hydrocortisone[32 mg/(kg.d)]in-tramuscularly every day,for consecutive 12 d to induce Kidney-Yang deficiency pattern POF model.After modeling,treatment groups were given relevant drugs intragastrically,and normal group and model group were given purified water intragastrically,twice a day,morning and afternoon,for consecutive 14 d.The changes of body weight,food consumption,ovary index,uterus in-dex,estrous cycle and serum reproductive hormone(E2,FSH,LH)were observed in each group.RESULTS:At the end of admin-istration,compared with normal group,body weight,ovary index and serum level of E2 decreased and estrous cycle disturbance was found in model group;while the levels of FSH and LH in serum,FSH/LH ratio increased(P〈0.05 or P〈0.01).Compared with model group,estrous cycle disturbance,ovary index and serum reproductive hormone level were improved significantly in epimedium group and L.meyenii powder high-dose group(P〈0.05).Only ovary index and serum level of LH were improved significantly in L.meyenii powder low-dose group(P〈0.05),there was no statistical significance in other index(P〉0.05).CONCLUSIONS:High-dose L.meyenii powder could promote the recovery of ovary function and the estrous cycle,and improve the ovary index and serum reproductive hormone level in POF model rats with Kidney-Yang deficiency pattern.It shows certain effect on the maintenance of ovarium.
作者
王凯
刘帅帅
郭起岳
林基伟
吴知凡
孙晓生
WANG Kai;LIU Shuaishuai;GUO Qiyue;LIN Jiwei;WU Zhifan;SUN Xiaosheng(Guangzhou University of TCM,Guangzhou510006,China;Institute of Liu Minru’TCM Gynecology,Guangdong Shenzhen 518000,China;Shenzhen San-jiu Outpatient Department,Guangdong Shenzhen 518000,China;Guangdong Medical Laboratory Animal Center,Guangdong Foshan 528248,China)
出处
《中国药房》
CAS
北大核心
2016年第28期3929-3932,共4页
China Pharmacy
基金
广东省教育部产学研结合基金项目(No.2012B091-100485)
关键词
玛咖粉
肾阳虚证
卵巢早衰
养护
大鼠
Lepidium meyenii powder
Kidney-Yang deficiency pattern
Premature ovarian failure
Maintenance
Rats
