摘要
目的:建立同时测定左归丸药液中没食子酸、原儿茶酸、绿原酸、马钱苷、阿魏酸5种化学成分含量的分析方法。方法:采用HPLC-DAD多波长切换-梯度洗脱技术。色谱柱:Eclipse XDB-C_(18)(4.6 mm×250 mm,5μm);流动相:甲醇-1%醋酸水溶液,梯度洗脱;流速:1.0 m L·min^(-1);分别以各成分的最大吸收波长为检测波长;柱温:30℃。结果:左归丸药液中没食子酸、原儿茶酸、绿原酸、马钱苷、阿魏酸5种成分均具有良好的线性关系(r≥0.999),平均加样回收率为97.76%~101.8%(RSD<3%,n=6)。3批样品中没食子酸、原儿茶酸、绿原酸、马钱苷、阿魏酸含量分别为0.489 0~0.500 6、0.104 4~0.108 4、0.048 56~0.050 66、1.042~1.061、5.287×10-3~5.369×10^(-3) mg·m L^(-1)结论:该方法经方法学验证,可用于左归丸药液及其制剂的质量控制。
Objective： To establish a method for simultaneous determination of gallic acid, protocatechuic acid, chlorogenic acid, loganin and ferulic acid contents in herb liquor of Zuogui pills. Methods： HPLC-DAD and the gradient elution wavelength shifts technique were used in the study. The column was Eclipse XDB-C18 （ 4. 6 mm × 250 mm, 5 μ m ） ; the mobile phase was methanol-1% acetic acid solution at the flow rate of 1 mL· min^-1, the detection wavelength was the maximum absorption wavelength of each ingredient; the oven temperature was 30 qC. Results： The contents of all 5 indicative components in herb liquor of Zuogui Pill had a good linearity （ r t〉 0.999 ）. The average recoveries were 97.76%-101.8% （RSD〈3%, n=6 ）. The contents of gallic acid, protocateehuie acid, chlorogenic acid, loganin and ferulic acid in 3 samples were 0. 489 0-0. 500 6 mg· mL^-1, 0.104 4-0.108 4 mg· mL^-1, 0.048 56-0.050 66 mg·mL^-1, 1.042-1.061 mg· mL^-1, 5.287 × 10^-3-5.369 × 10^-3 mg· mL^-1, respectively. Conclusion： This method has been validated, so it can be used to control the quality of herb liquor of Zuogui Pill and its preparation.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2016年第8期1364-1369,共6页
Chinese Journal of Pharmaceutical Analysis
基金
2012年国家自然科学基金项目(批准号:81273772)
关键词
左归丸
没食子酸
原儿茶酸
绿原酸
马钱苷
阿魏酸
熟地
山药
枸杞
山茱萸
川牛膝
鹿角胶
龟板胶
菟丝子
高效液相色谱法
Zuogui pills
gallic acid
protocatechuic acid
chlorogenic acid
loganin
ferulic acid
Radix Rehmanniae
Rhizom Dioscoreae
Fructus Lycii
Fructus Corni
Radix Cyathulae
Colla Cornu Cervi
Colla Carapax et Plastrum Testudinis
Semen Cuscutae
HPLC
