摘要
目的:探讨产前应激对雄性子代大鼠大脑中动脉缺血/再灌注后星形胶质细胞的影响。方法:SD孕鼠随机分为有产前应激处理(妊娠第15到21天每日3次限制活动)和无产前应激处理,并对其雄性子代大鼠采用线栓法制备大脑中动脉闭塞(MCAO)模型,共分为产前应激+假手术组、MCAO模型组、产前应激+MCAO组(n=10),于再灌注后第5天检测脑梗死体积,免疫荧光双标染色检测缺血灶边缘区星形胶质细胞形态及促红细胞生成素肝细胞受体A4(EphA4)和胶质纤维酸性蛋白(GFAP)的共表达情况,并采用Western blot检测EphA4、GFAP和神经蛋白聚糖(Neurocan)蛋白表达。结果:产前应激+MCAO组子代大鼠脑梗死体积百分比、EphA4、GFAP和Neurocan蛋白表达均较MCAO组显著增加(P均<0.05),且GFAP阳性细胞形态学改变及EphA4/GFAP共表达也较MCAO组明显。结论:产前应激可能改变子代大鼠脑缺血/再灌注后星形胶质细胞上EphA4受体的表达,促进星形胶质细胞活化,产生神经蛋白聚糖。
Objective： To investigate the effects of prenatal stress on astrocytes after ischemia/reperfusion of cerebral middle artery in adult offspring rats. Methods： Pregnant rats were randomly assigned to prenatal stress treatment group, which was exposed to restraint three times daily in the last week of pregnancy, and no prenatal stress treatment group. Adult male offspring rats were subjected to transient focal cerebral ischemia by middle cerebral artery occlusion （MCAO）. There were three groups： prenatal stress ＋ sham group, MCAO group and prenatal stress ＋ MCAO group （ n = 10）. After 5 days of reperfusion, the infarct size was evaluated. The morphology of astrocytes, co-localization of erythropoietin-produeing hepatocellular receptor A4 （EphA4） and glial fibrillary acidic protein （GFAP） were detected by double immunofluorescent staining. And the protein expressions of EphA4, GFAP and Neurocan in peri-ischemic regions were detected by Western blot. Results： The infarct size and the expression of EphA4, GFAP and Neuroean were significantly increased in prenatal stress ＋ MCAO group compared with MCAO group （all P 〈 0.05）. And the morphological changes of GFAP-posifive astrocytes and co-localization of EphA4/GFAP were more obvious in prenatal stress ＋ MCAO group compared with MCAO group. Conclusion： Prenatal stress may upregulate the expression of EphA4 on astrocytes in the offspring rats after cerebral ischemla/reperfusion, which promotes the reactivity of astrocyte and increases the expression of neurocan.
出处
《中国应用生理学杂志》
CAS
CSCD
2016年第4期301-304,309,共5页
Chinese Journal of Applied Physiology
基金
福建省自然科学基金项目(2015J01449)
福建省教育厅项目(JB13067)
泉州市技术研究与开发项目(2012Z35)
院苗圃基金(2012MP65)
