摘要
高架十字迷宫(the elevated plus maze,EPM)是研究小鼠焦虑行为的重要实验,本文介绍了EPM的原理和实验步骤。将小鼠置于开臂闭臂接合处,面向开臂,通过录像记录分析小鼠在EPM的表现。结果发现,两种小鼠均可成功完成实验,C57小鼠在EPM内较不活跃,闭臂滞留时间占总时间百分比显著高于129Sv小鼠。应用EPM可以简单直观地分析小鼠焦虑行为。
The elevated plus maze （EPM） is an important behavioral assay for rodents to assess the anxi- ety behavior. We introduced the basic principle and protocol of EPM. Mice are placed at the junction of the four arms facing the same open arm, activities were recorded by a video-tracking system. Both finished the test, but C57 showed less general activity than 129Sv. C57 mice showed significantly higher percent of closed/total time than the 129Sv. EPM is a simple and direct test to analyze anxiety behavior in mice.
出处
《中国细胞生物学学报》
CAS
CSCD
2011年第5期466-472,共7页
Chinese Journal of Cell Biology
基金
国家863计划(No.2008AA02Z126)
上海市科委科技创新行动计划(No.10140901400)资助项目~~
关键词
高架十字迷宫
焦虑
行为学
小鼠
the elevated plus maze
anxiety
ethology
mouse
