摘要
目的通过观察大鼠大脑中动脉缺血再灌注损伤后7 d,大鼠神经功能、生长相关蛋白-43(GAP-43)、突触素(SYN)表达的变化,探讨经颅重频磁刺激(rTMS)对缺血性损伤后脑组织可塑性的影响。方法制作大鼠右侧大脑中动脉梗塞再灌注模型,连续7 d给予不同频率和强度的rTMS,同时设立假刺激组和假手术组进行对照。观察大鼠神经功能的变化,并应用免疫组化和Western blot技术检测脑组织GAP-43、SYN的表达。结果 rTMS组大鼠神经功能缺损评分较对照组明显降低,病灶侧脑组织内GAP-43、SYN蛋白水平较对照组显著增高。高频高强rTMS组与其他组差异显著(P<0.05)。结论连续rTMS可以增强神经重塑作用,促进神经功能恢复。
Objective To explore the effect of rTMS on the plasticity of the brain after ischemic injury.MethodsSD rat MCAO-reperfusion model was established and then divided into four groups.Different rTMS were applied within 7 days after the reperfusion.The neurological impairment evaluation score of rats were recorded during this period.Immunohistochemistry and Western blot techniques were used to detect GAP-43 and SYN protein expressions.Results rTMS improved rehabilitation of neurofunction.rTMS increased the protein expression levels of GAP-43 and SYN.The therapeutic effect of high frequency and high intensity of rTMS were statistically better than other groups（P〈0.05）.Conclusion The application of rTMS may enhance the plasticity of the brain and improve neurofunction.
出处
《基础医学与临床》
CSCD
北大核心
2011年第1期1-5,共5页
Basic and Clinical Medicine
基金
国家自然科学基金(30470617)
关键词
经颅重频磁刺激
可塑性
生长相关蛋白-43
突触素
repetitive transcranial magnetic stimulation
plasticity
growth associated protein-43（GAP-43）
synaptophysin（SYN）
