摘要
为了揭示铁皮石斛内生真菌与功效成分的关系,该研究利用HPLC和紫外分光光度法测定21种铁皮石斛内生真菌菌丝及发酵液提取物中糖类和黄酮类成分。结果表明:21个供试菌株发酵产物乙酸乙酯提取物中均可检测到黄酮类成分,对比色谱保留时间和紫外光谱特征,发现DO49可产柚皮素,DO23,DO81,DO83可产芦丁;21个供试菌株发酵产物水提取物中均检测到多糖,但不同菌株单糖的组成存在差异,参照单糖种类以及甘露糖/葡萄糖的峰面积比,筛选与铁皮石斛多糖相关的优良菌株DO23,DO26,DO81,DO54,DO55,DO83。综合糖类和黄酮类两类成分,筛选出优良菌株DO23,DO81,DO83,既能产生与铁皮石斛相同的黄酮成分,又能产生单糖组分与铁皮石斛相近的多糖。
To reveal the relationship between endophytic fungi and the functional components,saccharides and flavonoids in the mycelia or fermented liquor of 21 endophytic fungi in D. officinale were detected using HPLC and UV spectrophotometer. The results showed that the ethyl acetate extracts from 21 fungal strains all contain flavonoids. According to the chromatographic retention time of HPLC and UV spectra characteristics of flavonoids,strain DO49 was found produce naringenin,strains DO23,DO81 and DO83 were found produce rutin. The water-soluble extracts from 21 strains all had polysaccharides. However,there was difference in the composition of monosaccharides derived from polysaccharides among different strains. According to the composition of monosaccharides and the peak area ratio of mannose and glucose,the fungal strains including DO23,DO26,DO81,DO54,DO55,DO83 product polysaccharides associated with D. officinale were selected. In conclusion,based on the saccharides and flavonoids,the excellent endophytic fungal strains DO23,DO81 and DO83 were selected,which could produce the same flavonoids and similar polysaccharides in D. officinale.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2016年第12期2208-2212,共5页
China Journal of Chinese Materia Medica
基金
浙江省林学重中之重一级学科创新计划项目(201523)
杭州市重大科技专项(20142012A36)
浙江农林大学人才启动基金项目(2012FR077)
关键词
铁皮石斛
内生真菌
单糖组成
黄酮成分
HPLC
Dendrobium officinale
endophytic fungi
monosaccharide composition
flavonoids ingredients
HPLC
