摘要
为揭示初筛所得7株活树附生铁皮石斛内生真菌对宿主生长及代谢成分的影响,该研究观察测量了接菌与未接菌铁皮石斛组培苗形态特征与农艺性状,采用苯酚-硫酸法与热浸法测定组培苗茎总多糖含量与浸出物含量,柱前衍生HPLC分析茎多糖中单糖组成,HPLC分析茎醇溶性成分。研究表明,供试7个菌株可改变铁皮石斛组培苗茎节变紫进程,并影响茎糖类及醇溶性成分组成及含量积累,除DO34外均能促进组培苗生长与提高多糖含量,除DO12外均能促进甘露糖含量积累,DO18,DO19,DO120可促进醇溶性浸出物含量积累,综合生长及代谢成分多项指标筛选出DO14,DO18,DO19与DO120 4个优良菌株,为铁皮石斛内生真菌和宿主相互作用机制研究与微生物工程研究提供了菌种材料与研究基础。
The paper aims to study the effects of endophytic fungi from D. officinale cultivated on living trees on growth and components metabolism of tissue culture seedlings. Morphological characteristics and agronomic characters of tissue culture seedlings infected and uninfected by endophytic fungus were observed and measured. Polysaccharides and alcohol-soluble extracts contents were determined by phenol-sulfuric acid method and hot-dipmethod,respectively. Monosacchride composition of polysaccharides and alcohol-soluble extracts components were analyzed by pre-column derivatives HPLC and HPLC method,respectively. It showed that effects of turning to purple of stem nodes could be changed by endophytic fungus. Besides,the endophytic fungus could affect the contents and constitutions of polysaccharides and alcohol-soluble extracts. The strains tested,expect DO34,could promote growth and polysaccharides content of tissue culture seedlings. The strains tested,expect DO12,could promote the accumulation of mannose. Furthermore,DO18,DO19 and DO120 could increase alcohol-soluble extracts. On the basis,four superior strains were selected for mechanism research between endophytic fungus and their hosts and microbiology engineering.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2016年第9期1602-1607,共6页
China Journal of Chinese Materia Medica
基金
浙江省重大科技专项(2013C02011)
关键词
铁皮石斛
内生真菌
生长发育
代谢成分
Dendrobium officinale
endophytic fungi
growth and development
components metabolism
