摘要
目的测定17个产地木芙蓉Hibiscus mutabilis Linn叶中芦丁的含有量。方法收集17份野生木芙蓉种质资源,考察其叶主要生长性状(叶柄长、叶片长、叶片宽、叶长宽比)。HPLC法测定叶片芦丁含有量,分析其与种源、经纬度、海拔、生长性状间的相关性,并进行聚类分析。结果供试木芙蓉种源间芦丁含有量存在极显著差异(P<0.01),平均含有量为1.389 6 mg/g,其中浙江、云南、湖北、广西种源芦丁含有量相对较高。芦丁含有量与海拔呈正相关关系,与经度、叶柄长、叶长宽比呈负相关关系,与纬度相关性较低。17个种源可划分为4个类群,其中类群Ⅰ、Ⅱ种源芦丁含有量较高。结论该方法准确稳定,重复性好,可用于木芙蓉的质量控制。
AIM To determine the rutin contents in Hibiscus mutabilis Linn leaves from seventeen growing are-as.METHODS Seventeen germplasms of Hibiscus mutabilis were collected from the main distrilDution areas and their growth characteristics including length of stipe and leaves,width of leaves,ratio of length and width of leaves were studied.The contents of rutin were determined by HPLC,and their correlations witli altitude,longitude,lati-tude,growtli characteristics and germplasms were analyzed,then cluster analysis was made.RESULTS There were significant diferences of growt!i characteristics and rutin content among diferent germplasms of H.mutabilis(P<0.01),witii 1.389 6 mg/g as their mean value,those collected from Zhejiang,Yunnan,Hubei and Guangxi were relatively higher than others.Moreover,the rutin content had a positive correlation with altitude,a negative correlation with longitude,length of stipeand shapeindex of leaves,and no significant correlations with latitude.Meanwhile,seventeen germplasms could be divided into four groups.Those from group I and*had higher rutin contents.CONCLUSION This accurate,stable and reproducible method can beused for thequality control of H.mutabilis.
作者
王曦林
李王武
邹慧龙
WANG Xi-lin;LI Wang-wu;ZOU Hui-long(Lishui People's Hospital,Lishui 323000,China)
出处
《中成药》
CAS
CSCD
北大核心
2018年第4期886-890,共5页
Chinese Traditional Patent Medicine
基金
浙江省科技计划项目(2015CXTD11)
关键词
木芙蓉
叶
芦丁
HPLC
聚类分析
Hibiscus mutabilis Linn
leaves
rutin
HPLC
cluster analysis
