摘要
目的:研究磁性纳米材料搭载喜树碱(MNC-Camp)的制备、药物释放、细胞吸收及抗肺癌作用。方法:紫外分光光度法监测MNC-Camp的药物释放能力,普鲁兰染色法检测A549肺癌细胞对磁性纳米材料的吸收能力的影响,利用MTT法测定游离Camp和MNC-Camp对A549细胞株和白细胞生长的抑制能力,细胞侵袭法分析游离Camp和MNC-Camp对A549细胞转移能力的影响,酶标仪吸光度法检测细胞凋亡蛋白caspase-3的活性。结果:MNC-Camp的药物释放随着时间的增加而增加,A549肺癌细胞对磁性纳米材料具有良好的吸收能力;与MNC组比较,游离Camp和MNC-Camp能够使A549细胞生长能力降低,IC_(50)分别为(35.14±3.21)和(7.16±2.54)mg/L,且A549细胞转移的数目下降,凋亡蛋白caspase-3活性增加,以上作用效果MNC-Camp明显强于游离Camp(P<0.05)。结论:磁性纳米材料搭载喜树碱比游离的喜树碱更能显著降低人肺癌A549细胞的生长和转移能力,提示磁性纳米材料能增强喜树碱的抗肺癌作用。
AIM： To study the preparation,drug release,cell absorption and anti-lung cancer efficacy of magnetic nanoscale carrier with camptothecin（ MNC-Camp）. METHODS： The drug release ability of MNC-Camp was detected by ultraviolet spectrophotometric method. Absorption of magnetic nanoparticles by A549 cells was measured by pullulan staining method. The growth inhibition ability of Camp and MNC-Camp on the A549 cells and white blood cells was analyzed by MTT assay. The metastasis ability of A549 cells treated with Camp and MNC-Cmap was evaluated by cell invasion method. The activity of apoptosis protein caspase-3 was detected by ELISA. RESULTS： A549 cells had a good absorption ability on MNC,and the drug release of MNC-Camp increased with time increasing. Compared with MNC group,Camp and MNC-Camp induced the apoptosis of A549 cells,and the half inhibition concentration（ IC_（50）） were（ 35. 14 ± 3. 21） and（ 7. 16 ± 2. 54） mg/L,respectively. Camp and MNC-Camp also decreased the A549 cell transfer number and increased the activity of apoptosis protein caspase-3. All the effects of MNC-Camp were obviously significant than those of free Camp（ P〈0. 05）. CONCLUSION： MNC-Camp has a better inhibitory effect on the growth of A549 cells than free Camp,and finally plays an important role in anti-lung cancer effect.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2016年第5期928-932,共5页
Chinese Journal of Pathophysiology
基金
河南省医学科技攻关计划(No.2011020091)
