摘要
目的:观察沙枣提取物对人肝癌Bel-7404细胞增殖抑制作用及其对细胞凋亡的影响。方法:以没食子酸为对照品,采用Folin-Ciocalteu法测定沙枣提取物各分离组分的总酚含量。通过MTT法,观察各组分对Bel-7404细胞增殖抑制作用。选取对Bel-7404增殖抑制活性最强的20%乙醇洗脱组分,设50、100、150μg/ml剂量组,采用Ainexin V-FITC/PI双染法,流式细胞仪检测其诱导细胞凋亡,并采用Hoechst 33258荧光染色法观察细胞形态变化。结果:沙枣提取物各分离组分对Bel-7404增殖抑制作用各不相同,其中20%乙醇洗脱组分的50、100、150μg/ml剂量组的凋亡率分别为29.8%、58.7%和76.2%;凋亡细胞的细胞核或细胞质内可见浓染致密的颗粒块状荧光。结论:沙枣提取物可抑制Bel-7404细胞增殖,诱导其凋亡,并与总酚含量呈现一定的量效关系。
Objective： To observe effect of Elaeagnus angustifolia extracts on proliferation and apoptosis of human hepatoma Bel-7404 cells. Methods： To use gallic acid as reference substance and determine total phenol contents of separated fractions of E. angustifolia extract by the Folin-Ciocalteu method. To observe proliferation effects of Bel-7404 cell by MTT assay. Selected 20% ethanol elution fraction which was the most active proliferation inhibition on Bel-7404 cell and analyzed apoptosis effects of different concentrations（ 50,100,150 μg / ml） by Ainexin V-FITC / PI double staining method. Cell morphology was observed by Hoechst33258. Results： Different separated fractions have different inhibited proliferation activities to Bel-7404 cell. Apoptosis rates of 20% ethanol fraction（ 50,100,150 μg / ml） were 29. 8%,58.7%,76. 2%,respectively. Fluorescent particles blocks were observed in the nucleus or cytoplasm by Hoechst33258 fluorescent dye staining. Conclusion： Elaeagnus angustifolia extracts can inhibit the cell proliferation and induce the apoptosis of human hepatoma Bel-7404 cells.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2016年第1期99-102,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
新疆维吾尔自治区自然科学基金(2012211A027)
