摘要
【目的】优化微波协同复合酶法提取甘孜虎掌菌多糖的工艺条件,为提高虎掌菌多糖提取率提供新的工艺技术。【方法】以甘孜虎掌菌为试验材料、多糖提取率为考察指标,在单因素试验基础上,通过Box-Behnken中心组合设计及响应面法优化微波协同复合酶法提取甘孜虎掌菌多糖的工艺条件。【结果】影响微波协同复合酶法提取甘孜虎掌菌多糖的因素顺序为:复合酶用量>酶解温度>微波功率>微波时间,复合酶用量和酶解温度对多糖提取率的影响极显著(P<0.01),酶解温度与微波时间两因素交互作用影响显著(P<0.05),其最佳提取工艺条件为:纤维素酶与木瓜蛋白酶的复合酶质量比1∶1、复合酶用量0.70%、p H 5.0、酶解温度51℃、微波时间4.3 min、微波功率550 W,在此条件下的虎掌菌多糖提取率为16.09%,与模型预测值16.2537%的相对误差为1.02%,误差较小。【结论】建立的模型对甘孜虎掌菌多糖具有较好的预测作用,优化的工艺参数可用于实际生产。
【Objective】The present exp eriment was conducted to optimize microwave-enzyme synergistic extraction process of polysaccharide from Sarcodon imbricatus in Ganzi, Sichuan, in order to provide new extraction process for improve extraction rate of polysaccharide from S. imbricatus. 【Method】Using S. imbricatus from Ganzi, Sichuan as materials and extraction rate of polysaccharide as indexes, the polysaccharide were extracted from S. imbricatus by microwave-enzyme synergistic method, based on single factor experiment, and the process conditions were optimized using Box-Behnken central complex design and response surface analysis method. 【Result】The results showed that, some factors affecting extraction rate of polysaccharide from S. imbricatus were ranked, as follows： complex enzyme dosage enzymolysis temperaturemicrowave powermicrowave time. Furthermore, the complex enzyme dosage and enzymolysis temperature had extremely significant effects on extraction rate of polysaccharide（P0.01）, and the interaction between enzymolysis temperature and microwave time had significant effects on extraction rate of polysaccharide（P0.05）. In addition, the optimum process conditions were as follows： cellulase and papaya proteinase at mass ratio of 1 ∶1 in complex enzyme, complex enzyme dosage of 0.70%, p H 5.0, microwave time of 4.3 min, microwave power of 550 W, enzymolysis temperature of 51 ℃.Under the above optimum conditions, the extraction rate of polysaccharide was 16.09%, and the model predicted value was 16.2537%, thus the relative error was 1.02%. 【Conclusion】The established model can effectively predict extraction efficiency of polysaccharide from S. imbricatus, and the optimized process parameter can be also applied in actual production.
出处
《南方农业学报》
CAS
CSCD
北大核心
2016年第3期442-448,共7页
Journal of Southern Agriculture
基金
四川医科大学青年基金项目(20140239)
关键词
虎掌菌
多糖
响应面法
微波协同复合酶法
甘孜
Sarcodon imbricatus
polysaccharide
response surface methodology
microwave-enzyme synergistic method
Ganzi
Sichuan
