摘要
目的优化刺葡萄皮多酚的提取工艺,测定刺葡萄皮中多酚物质的体外抗氧化活性。方法在单因素实验的基础上,通过正交试验优化超声辅助提取刺葡萄皮多酚的工艺,并通过与Vc(或EDTA)对比,测定其清除ABTS+自由基、DPPH自由基、OH自由基的能力,亚铁离子螯合能力及对大鼠脑匀浆脂质过氧化的影响,对刺葡萄皮多酚的抗氧化活性进行综合研究。结果超声辅助提取刺葡萄皮多酚的最佳工艺参数为:乙醇浓度40%,料液比1∶14,超声功率225W,提取时间40min;该条件下的提取量是16.823 mg/g。刺葡萄皮多酚的体外抗氧化试验表明其对ABTS+自由基、DPPH自由基有较强的清除能力,对清除OH自由基、亚铁离子的螯合和抑制大鼠脑匀浆脂质过氧化的能力较弱。结论本研究表明在该优化工艺下提取的刺葡萄皮多酚具有抗氧化活性,但是比Vc偏弱。
Objective Optimization the extraction technology of total phenolic compounds from the spine grape（Vitis davidii（Rom. Caill.） Foёx） peel,and determine its antioxidant activity. Methods The extraction technology of total phenolic compounds from the spine grape peel was studied by single-factor experiments and orthogonal test design. Furthermore,the antioxidant activities of spine grape peel were compared with Vc（or EDTA）,using various antioxidant assays included the scavenging on ABTS＋,DPPH and OH free radicals,ferrous ion-chelating capacity and influence on lipid peroxidation in rats. Results The optimum extraction conditions determined by orthogonal experiment as follows：ethanol concentration 40%,solid-liquid ratio was 1∶14,ultrasonic power225 W and extraction time 40 min. The extraction quantity of total phenolic compounds under this condition was 16. 823 mg/g. It has strong scavenging activity on ABTS＋and DPPH,while weak scavenging activity on OH free radicals,ferrous ion-chelating capacity and inhibiting the brain lipid peroxidation in rats. Conclusion This study suggested that spine grape peel has antioxidant activity under the optimal extraction technology,but it’s weaker than Vc.
出处
《云南中医学院学报》
2016年第1期21-26,共6页
Journal of Yunnan University of Traditional Chinese Medicine
基金
国家自然科学基金(81374048)
国家级大学生创新创业训练计划项目(201410344008)
浙江省大学生科技创新活动计划暨新苗人才计划(2014R410003)
浙江中医药大学2014年学生科研基金(浙中大团[2014]3号)
关键词
刺葡萄皮
多酚
提取
抗氧化
spine grape peel
total phenolic compounds
extraction
antioxidant activity
