摘要
目的研究葡萄籽多酚(GSP)逆转先天性耐药细胞株GBC-SD耐药的机制,寻找高效低毒的耐药逆转剂。方法选择先天性耐药细胞株GBC-SD为研究对象。MTT比色法测定各化疗药物的半数抑制浓度(IC50);RT-PCR测定MDR 1 mRNA的变化;流式细胞仪检测P-gp,bcl-2蛋白和细胞内阿霉素浓度的变化。结果(1)无毒(3μg/mL)和低毒(6μg/mL)浓度的GSP处理后各化疗药物的IC50值均明显下降(P<0.0 5),能明显逆转GBC-SD的多药耐药性;(2)上述两浓度的GSP能下调GBC-SD细胞MDR 1 mRNA表达(P<0.0 5);(3)上述两浓度的GSP能下调GBC-SD细胞P-gp和bcl-2蛋白表达(P<0.0 5);(4)GSP增加GBC-SD细胞内ADM药物浓度(P<0.0 5)。结论GSP能部分逆转先天性耐药细胞株GBC-SD多药耐药性,其作用机制为下调GBC-SD细胞MDR 1 mRNA,及P-gp和bcl-2蛋白表达。
Objective To explore the mechanism of reversal of mutidrug resistance of GBC-SD cell lines by grape seed polyphenols （ GSP ）. Methods GBC-SD cell lines were used to determine the effect of GSP. MTT assay was adopted to evaluate the cytotoxity （ IC50 ） , RT-PCR were used to determine MDR1 mRNA, P-gp, bcl-2 and cellular adriamycin was measured by flow cytometry. Results In non-toxic （3μg/mL ） and low toxic （6μg/mL） comcentration of GSP treated group （ P 〈 0. 05 ） , the IC50 of chemotherapeutic agents was reduced the expression of P-gp, bcl-2 and MDR1 mRNA inhibited （ P 〈 0. 05 ） , and cellular accumulation of adriamycin increased （ P 〈 0. 05 ）. Conclusions GSP could reverse mutidrug resistance of GBC-SD cell lines, and the suggested mechanism is that GSP decreased the expression of P-gp, bcl-2 and MDR 1 mRNA.
出处
《中国普通外科杂志》
CAS
CSCD
2006年第3期202-205,共4页
China Journal of General Surgery
关键词
胆囊癌细胞株
葡萄籽多酚
药理学
耐药性
Gallbladder Carcinoma Cell Line
Grape Seed Polyphenols/pharm
Mutidrug Resistance
