摘要
目的对腹泻住院患儿肠道腺病毒的核酸进行检测分析,以了解武威地区腹泻患儿肠道腺病毒的感染情况。方法收集2012年-2014年900例临床确诊病毒性腹泻患儿的粪便标本和临床资料,用逆转录PCR（RT-PCR）法检测腺病毒。结果 900例患儿中,腺病毒感染140例（15.56%）,2012年-2014年每年度腺病毒阳性检出率分别为10.33%、19.00%、17.33%,差异有统计学意义（χ^2=9.66,P〈0.05）。男性患儿腺病毒检出率较女性患儿高,腺病毒检出率与患儿年龄和发病时间有关。腺病毒检出患儿和腺病毒非检出患儿的日均腹泻和呕吐次数比较,差异有统计学意义（P〈0.05）,其他临床症状如发热程度、脱水等症状均相似。结论肠道腺病毒是感染武威地区婴幼儿腹泻的主要病原体之一,建立完善的腺病毒流行监测系统是当前预防与控制婴幼儿腹泻的重要环节。
Objective To detect and analyze nucleic about enterric adenovirus among children hospitalized with diarrhea so as to understand the infection situation of enterric adenovirus in Wuwei region. Methods 900 stool specimens and clinical data were collected from children with diarrhea in from 2012 to 2014. Reverse transcription- PCR（ RT- PCR） was employed to detect human adenovirus. Results In 900 children,140 cases（ 15. 56%） were infected with adenovirus. The positive detection rate were 10. 33%,19. 00% and 17. 33% from 2012 to 2014,respectively. The difference was statistically significant（ χ~2=9. 66,P〈0. 05）. The detection rate of adenovirus in male children patients was higher than that in female. The adenovirus detection rate associated with children age and period. Further more,the number of day diarrhea and emesis had statistically significant difference in infected and non- infected children group（ P〈0. 05）. Other symptoms such as heating degree,dehydration and other symptoms were simila. Conclusion Intestinal adenovirus infection is one of the main pathogens of infantile diarrhea in Wuwei region. Establishment and improvement of the epidemic monitoring system of adenovirus is an important link in the prevention and control of diarrhea in infants and young children.
出处
《中国卫生检验杂志》
CAS
2016年第3期429-431,共3页
Chinese Journal of Health Laboratory Technology
关键词
腺病毒
腹泻
婴幼儿
Enterric adenovirus
Diarrhea
Infants and young children
