摘要
目的通过对2021年6月甘肃省兰州市某幼儿园发生一起以呕吐、腹泻为主症的聚集性疫情,确定引起本次疫情的病原体。方法采集发病幼儿肛拭子、食堂、教室等环境标本共44份(17份患儿肛拭子标本和27份环境标本),运用聚合酶链式反应检测轮状病毒、诺如病毒、肠道腺病毒及札如病毒核酸,肛拭子进行致泻菌分离培养。结果44份标本未分离到致泻性大肠埃希菌、沙门菌、志贺菌、小肠结肠炎耶尔森菌等致病菌,轮状病毒、诺如病毒、星状病毒及肠道腺病毒核酸检测均为阴性,札如病毒核酸检测到患儿肛拭子阳性11份,其余标本均为阴性。选取其中2份强阳性标本进行全基因组测序,为札如病毒GⅠ.2型,基本局部比对搜索工具(BLAST)结果与2016年深圳市发现的札如病毒全序列相似度在98.96%~98.98%。结论此次聚集性疫情由札如病毒GⅠ.2型引起,是甘肃首次发现札如病毒引起的暴发疫情,提示应加强病毒性腹泻的监测和防控。
Objective To identify the pathogen of an epidemic of vomiting and diarrhea with unknown cause in a kindergarten in Lanzhou,Gansu province.Methods A total of 44 samples,including 17 anal swabs from sick children and 27environmental samples,were collected.The nucleic acids of rotavirus,norovirus,zavivirus and sapovirus were detected by real-time fluorescence polymerase chain reaction(RT-PCR),and the bacterial pathogen isolation was conducted by using the anal swabs.Results In the 44 samples,no diarrheagenic Escherichia coli,Salmonella,Shigella and Yersinia enterotica were isolated,and the nucleic acid detection results of rotavirus,norovirus,astrovirus and zavivirus were negative,but 11 children’s anal swabs were positive for the nucleic acid of sapovirus.Two anal swabs with obvious bands were selected for wholegenome sequencing,the results showed that the sapovirus belonged to GⅠ.2,and BLAST indicated that the similarity of the whole genome sequence with the sapovirus detected in Shenzhen in 2016 was 98.96%-98.98%.Conclusion The epidemic of diarrhea was caused by sapovirus GⅠ.2,which was first reported sapovirus infection epidemic in Gansu,indicating that it is necessary to strengthen the surveillance,prevention and control of viral diarrhea.
作者
汪鹏
王雪莹
康倩
于德山
Wang Peng;Wang Xueying;Kang Qian;Yu Deshan(Gansu Provincial Center for Disease Control and Prevention,Lanzhou 730000,Gansu,China)
出处
《疾病监测》
CAS
CSCD
北大核心
2023年第2期233-236,共4页
Disease Surveillance
基金
甘肃省青年科技基金计划(No.20JR5RA140)。
