摘要
以迷你文心兰侧芽为材料,系统研究了外植体取材与消毒,诱导、增殖、分化、生根培养基筛选等组培快繁技术,旨在为迷你文心兰种苗生产奠定技术基础。结果表明院采集当年新生侧芽,长4～6 cm,采用0.1%Hg Cl2溶液二次消毒的方法,有利于降低外植体污染率,提高成活率。类原球茎诱导以MS＋6-BA 4.0 mg/L＋NAA 1.0 mg/L＋蔗糖20 g/L效果最好,培养45 d最高诱导率达130%;增殖采用MS’＋6-BA1.0 mg/L＋NAA 0.2 mg/L＋蔗糖10 g/L附加5%苹果汁,培养60 d最高增殖倍数达40.7倍;分化采用MS’＋6-BA 0.8 mg/L＋NAA 0.1 mg/L＋蔗糖20 g/L,培养60 d,56.7%类原球茎可分化出芽;试管苗生根以MS’＋NAA 0.8 mg/L＋蔗糖20 g/L附加5%香蕉泥效果最好,培养60 d,平均生根数3.4条,平均根长3.16 cm。
Taking Mini Onc idium bud as material,the explant and disinfection induced,proliferation,differentiation,rooting culture medium screening technology of tissue culture and rapid propagation were systematic studied,to laid the technical foundation of mini Oncidium seedling production. The results showed that acquisition when newborn buds, ca. 4~6 cm,with 0.1% HgCl2 solution secondary disinfection method, could reduce the rate of explants pollution ,improve the survival rate. Effect of protocorm like body induction to MS＋6-BA 4.0 mg/L＋NAA 1.0 mg/L＋sucrose 20 g/L was the best ,culture 45 days with the highest induction rate of 130% ;proliferation by MS＇＋6-BA 1.0 mg/L＋NAA 0.2 mg/L＋sucrose 10 g/L added with 5% apple juice, culture 60 d the highest proliferation multiple was 40.7 times;differentiation using MS＇＋6-BA 0.8 mg/L＋NAA 0.1 mg/L＋suerose 20 g/ L,cultivated for 60 d, 56.7% of protocorm like bodies could bud differentiation;effect of rooting in MS＇ ＋NAA 0.8 mg/L＋sucrose 20 g/L added with 5% banana mud was best, medium 60 days, average root number was ＂3.4 and the average root length was 3.16 cm.
出处
《现代农业科技》
2016年第3期181-183,共3页
Modern Agricultural Science and Technology
基金
广东省科技计划项目(2013B020410002)
关键词
迷你文心兰
组培快繁
类原球茎
mini Oncidium
micropropagation
protocorm-like body
