摘要
以蝴蝶兰(Phalaenopsis)的花梗侧芽及花序顶端为外植体,在Kyoto或MS+BA3ppm培养基中诱导出营养芽。以试管植株的茎尖、茎段、叶片在MS+BA0.5-5ppm或MS+BA0.5-5ppm+NAA1ppm的培养基中,诱导出不定芽或原球茎状体(Protocorm-Like-Body,PLB.)。在附加BA_5+NAA_1的培养基里,茎段不定芽诱导率为65％,叶片PLB诱导率为16.7％,用MS+BA1ppm进行PLB继代培养可获得大量群体,进一步培养可形成完整小苗。 由蝴蝶兰花梗侧芽而来的白花无性系品种[Phal.(phyllis key ×bandleader)× Celebration]已大量出瓶移栽。在广州地区,蝴蝶兰出瓶苗种植2-3年即可开花。
Rapid clonal propagation of Phalaenopsis in vitro was studied.The vegetative buds were induced from lateral buds and the apices of Phalaenop-sis flower stalks in kyoto or Ms 3ppm BA medium. Shoot tips, stem sections and young leaves from in vitro plantlets formed protocorm-like-bodies (PLB) or adventitious buds in MS + 0.5-5ppm BA + lppm NAA or MS + 0.5-5ppm BA medium. When 5ppm BA + lppm NAA was added into the medium,65% of the stern sections formed adventitious buds,and 16.7% of the leaves formed PLB. In the MS + 1ppm BA medium, PLB sub-culture gave multiplication, and the structures formed young plants by further culture.
The plantlets of the white flower clone Phal. ( phyllis key × band lea-der) × celebration have been transplanted on a large scale. The study has also shown that plantlets of Phalaenopsis developed by this method start flowering in 2-3 years after transplantation in Guangzhou area.
出处
《园艺学报》
CAS
CSCD
北大核心
1989年第1期73-77,共5页
Acta Horticulturae Sinica
