摘要
目的:建立丹参中吡虫啉和多杀菌素的检测方法,并对残留动态情况进行对比研究。方法:样品用有机溶剂提取,利用固相萃取小柱净化,HPLC-DAD进行检测。结果:2种农药残留的前处理均采用固相萃取法,净化较为彻底,检测方法灵敏度高,重复性好,准确度、精密度和检出限均符合农残测定要求。消解动态数据的拟合结果表明,以2 h残留量为初始残留量,吡虫啉和多杀菌素半衰期(t1/2)分别为3.65 d和1.64 d,表明施药初期多杀菌素消解速度比吡虫啉快;1 d后吡虫啉和多杀菌素在丹参中的消解曲线符合一级动力学方程,t1/2分别为6.93 d和11.55 d,表明多杀菌素的持效期更长;在相同剂量标准下,多杀菌素的残留量均低于吡虫啉,因此多杀菌素的安全间隔期比吡虫啉更短。吡虫啉在土壤和产品中的最终残留分别为0.02,0.18 mg·kg-1,多杀菌素均未检出(<1.9μg·kg-1),表明多杀菌素的最终残留更低。结论:在丹参种植中,多杀菌素可作为一种替代吡虫啉的理想农药。
Objective： To establish methods for determining residual of imidacloprid and spinosad in Salviae Miltiorrhizae Radix et Rhizoma, and investigate dynamic residual of these two pesticides. Method：Samples were extracted with organic solvents,purified by solid phase extraction cartridges and detected by HPLCDAD. Result： These two pesticides were pretreated by solid phase extraction（ SPE） method,and purified more thoroughly. Detection methods had high sensitivity, good reproducibility, accuracy, precision and detection limits were in line with requirements of determination of pesticide residues. Degradation dynamics data showed that considering residues of 2 h as initial residues,half-life periods（ t1 /2） of imidacloprid and spinosad were 3. 65 d and1. 64 d. It showed that spinosad degraded faster than imidacloprid in the beginning. After one day,degradation curves of imidacloprid and spinosad in Salviae Miltiorrhizae Radix et Rhizoma consistented with first-order kinetic equations,t1 /2of them were 6. 93 d and 11. 55 d. It showed that spinosad had a longer duration of pesticide. At the same dosage level of pesticide,residue of spinosad was lower than imidacloprid,it showed that safe periods of spinosad was shorter than imidacloprid. The final residues of imidacloprid in soil and product were 0. 02 mg·kg- 1and 0. 18 mg·kg- 1,and spinosad was not detected（ 1. 9 μg·kg- 1） which showed that the final residue of spinosad was lower. Conclusion： It is concluded that in Salviae Miltiorrhizae Radix et Rhizoma planting,spinosad can be an ideal alternative pesticide to imidacloprid.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第3期28-31,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
山东省中医药科技发展计划项目(2011-174)
关键词
丹参
吡虫啉
多杀菌素
半衰期
农药残留
Salviae Miltiorrhizae Radix et Rhizoma
imidacloprid
spinosad
half-life
pesticide residues
