摘要
BACKGROUND： One of the major limitations of biliary stents is the stent occlusion, which is closely related to the over- growth of bacteria. This study aimed to evaluate the feasibility of a novel silver=nanoparticle-coated polyurethane （Ag/PU） stent in bacterial cholangitis model in swine. METHODS： Ag/PU was designed by coating silver nanopar- tides on polyurethane （PU） stent. Twenty-four healthy pigs with bacterial cholangitis using Ag/PU and PU stents were ran- domly divided into an Ag/PU stent group （n=12） and a PU stem group （n=12）, respectively. The stents were inserted by standard endoscopic retrograde cholangiopancreatography. Laboratory assay was performed for white blood cell （WBC） count, alanine aminotransferase （ALT）, interleukin-1 [l （IL- 1 p）, tumor necrosis factor-a （TNF-~） at baseline time, 8 hours, 1, 2, 3, and 7 days after stent placements. The segment of bile duct containing the stent was examined histologically ex vivo. Implanted bili- ary stents were examined by a scan electron microscope. The amount of silver release was also measured in vitro. RESULTS： The number of inflammatory cells and level of ALT, IL-1β and TNF-α were significantly lower in the Ag/PU stent group than in the PU stent group. Hyperplasia of the mucosa was more severe in the PU stent group than in the Ag/PU stent group. In contrast to the biofilm of bacteria on the PU stent, fewer bacteria adhered to the Ag/PU stent. CONCLUSIONS： PU biliary stents modified with silver nanoparticles are able to alleviate the inflammation of pigs with bacterial cholangitis. Silver-nanoparticle-coated stents are resistant to bacterial adhesion.
BACKGROUND： One of the major limitations of biliary stents is the stent occlusion, which is closely related to the over- growth of bacteria. This study aimed to evaluate the feasibility of a novel silver=nanoparticle-coated polyurethane （Ag/PU） stent in bacterial cholangitis model in swine. METHODS： Ag/PU was designed by coating silver nanopar- tides on polyurethane （PU） stent. Twenty-four healthy pigs with bacterial cholangitis using Ag/PU and PU stents were ran- domly divided into an Ag/PU stent group （n=12） and a PU stem group （n=12）, respectively. The stents were inserted by standard endoscopic retrograde cholangiopancreatography. Laboratory assay was performed for white blood cell （WBC） count, alanine aminotransferase （ALT）, interleukin-1 [l （IL- 1 p）, tumor necrosis factor-a （TNF-~） at baseline time, 8 hours, 1, 2, 3, and 7 days after stent placements. The segment of bile duct containing the stent was examined histologically ex vivo. Implanted bili- ary stents were examined by a scan electron microscope. The amount of silver release was also measured in vitro. RESULTS： The number of inflammatory cells and level of ALT, IL-1β and TNF-α were significantly lower in the Ag/PU stent group than in the PU stent group. Hyperplasia of the mucosa was more severe in the PU stent group than in the Ag/PU stent group. In contrast to the biofilm of bacteria on the PU stent, fewer bacteria adhered to the Ag/PU stent. CONCLUSIONS： PU biliary stents modified with silver nanoparticles are able to alleviate the inflammation of pigs with bacterial cholangitis. Silver-nanoparticle-coated stents are resistant to bacterial adhesion.
基金
partially supported by grants from the Jiangsu Province Social Development Program(BL2012031)
the National Natural Science Foundation of China(81172266)
the Natural Science Foundation of Jiangsu Province(BK2011859)
Jiangsu Innovation of Medical Team and Leading Talents Cultivation(LJ201127)
