摘要
目的建立东风桔药材HPLC指纹图谱,结合化学计量手段,对多批次药材进行质量控制。方法 采用Kromasil 100-C18（250 mm×4.6 mm,5μm）为色谱柱,以乙腈（A）-0.5%冰乙酸水（B）为流动相系统梯度洗脱（0-30 min,20%→50%A;30-70 min,50%→100%A）,流速为1.0 ml/min,检测波长为254 nm,柱温35℃。对12批药材样品进行相似度分析、聚类分析和主成分分析。结果 建立了东风桔药材专属性的HPLC指纹图谱,标示了18个共有指纹峰,12批样品分为5大类。结论 将HPLC指纹图谱与化学计量手段相结合,可有效对东风桔药材进行真伪鉴别和质量评价,为其质量控制方法提供参考。
Objective To establish the HPLC fingerprint chromatogram of Atalantia buxifolia coupled with chemometrics means for the quality control of multi-batches of medicinal material. Methods The samples were analyzed on Kromasil 100-C18（250 mm×4.6 mm,5 μm） column with a acetonitrile（A）-0.5% acetic acid（B） as the mobile phase（0-30 min,20%→50%A;30-70 min,50%→100%A） at a flow rate of 1.0 ml/min.The detective wavelength was 254 nm and the column temperature was at 35℃.12 samples was constructed for similarity evaluation,cluster analysis and principle component analysis. Results A specificity HPLC fingerprint spectrum was developed for Atalantia buxifolia,18 common peaks were indicated and 12 samples were divided into 5 groups. Conclusion With the combination of chemometrics means,the HPLC fingerprint chromatogram provides a method for evaluation of authenticity and quality control of Atalantia buxifolia,which is favorable to improve quality control of Atalantia buxifolia.
出处
《中国当代医药》
2016年第2期14-17,共4页
China Modern Medicine
基金
国家科技重大专项课题(2013ZX09201019)
关键词
东风桔
高效液相色谱法
指纹图谱
相似度评价
聚类分析
主成分分析
Atalantia buxifolia
HPLC
Fingerprint
Similarity evaluation
Cluster analysis
Principle component analysis
