摘要
目的 研究阿魏酸钠（ＳＦ）对庆大霉素（ＧＭ）肾毒性的保护作用。方法 采用近端肾小管上皮细胞原代培养技术制作ＧＭ损伤模型，观察细胞丙氨酸氨基转换酶（ＡＬＴ）、过氧化氢酶（Ｃａｔ）、ＮＡＧ酶活性，ＤＮＡ合成，细胞内离子钙浓度以及形态学变化，同时观察ＳＦ的保护作用。结果 ＧＭ组在现为ＡＬＴ，Ｃａｔ酶活性下降，ＮＡＧ酶活性增加，细胞ＤＮＡ合成下降，胞内游离钙浓度升高。光镜发现近端小管上皮细胞内有大量空泡变性和溶酶体髓样体形成。给予 ＳＦ能使上述异常指标明显改善，组织学损害也明显减轻。结论 ＳＦ能有效保护ＧＭ对肾小管上皮细胞的损害。
Aim To investigate the effect of sodium ferulate(SF), a compound derived from Chinese herbs, on gentamiein (GM) nephrotoxicity in renal tubular cells. Methods Alanine aminopeptidase (ALT), catalase (Cat), and N - acetyl -β- glucosaminidase (NAG) activi- ties, DNA synthesis, cytosolic free calcium concentration, and morphological alterations were observed in gentamicin - induced damage to cultured primary renal proximal tubular cells. The protection of SF to damages was observed too. Results In GM group, ALT and Cat activ- ities decreased, NAG activity invreased; besides, DNA synthesis decreased and cytosolic free calcium concentration increased. Electronmicrosope observation revealed an extensive vac- uolization in proximal tubular cells and formaion of myeloid bodies in lysosome. Simultaneous administration of SF with GM could markedly improve the values of parameters mentioned above and the histological damages. Conclusion SF can protect renal proximal tubular cell from GM nephrotoxicity.
