摘要
不同的发酵工艺会造成茶样中γ-氨基丁酸含量的不同．建立了全自动氨基酸分析仪快速测定乌龙茶样品中γ-氨基丁酸含量的方法，并测定不同发酵工艺的乌龙茶样品中γ-氨基丁酸的含量．茶样经浸泡后，过膜，使用全自动氨基酸分析仪进行测定．实验结果显示，方法检出限达到0．01g／100g，回收率为94．4％-109．4％，符合检测要求．方法前处理简单、快速，检测时间短，节省溶剂，测定结果准确、可靠，能够满足茶样中γ-氨基丁酸的日常检测工作，适用于大量样品的定性定量分析，对优化生产高含量γ-氨基丁酸的发酵工艺具有重要意义．
Different fermentation processes will result in different content of γ-aminobutyric acid in tea samples. A method for the determination of γ-aminobutyric acid in Oolong Tea using an automatic amino acid analyzer has been developed, and the trend for the content variation of γ-aminobutyric acid in Oolong Tea using different fermentation processes was investigated. Oolong Tea was soaked for some time, and then determined on a automatic amino acid analyzer. The detection limit of the method was 0.01 g/100g, and the recoveries ranged from 94.4% - 109.4% , which meet the requirements of determination. The method with a fast pretreatment procedure has a high throughput and is suitable for the quantitative and qualitative analysis ofγ-aminobutyric acid in Oolong Tea, and can be used to investigate different fermentation processes. The determination method is accurate and reliable, which meets the requirements of daily inspection. It also has great significance for the evaluation of the fermentation process whether it can produce high content of γ-aminobutyric acid.
出处
《分析测试技术与仪器》
CAS
2015年第4期231-235,共5页
Analysis and Testing Technology and Instruments
关键词
Γ-氨基丁酸
全自动氨基酸分析仪
乌龙茶
发酵工艺
γ-aminobutyric acid
automatic amino acid analyzer
Oolong Tea
fermentation process
