摘要
以铁线莲Clematis‘Bill MacKenzie’优良单株的嫩茎为外植体,研究了不同灭菌时间、基本培养基类型对C.‘Bill MacKenzie’愈伤组织诱导的影响,并在初代愈伤诱导的基础上,研究了不同外源激素组合对C.‘Bill MacKenzie’愈伤组织诱导及状态的影响。结果表明:最佳的灭菌时间为12min;基本培养基类型为MS,诱导率为68.4%;不同的外源激素组合均能诱导出愈伤,但是愈伤状态不同,其中MS+1.0mg/L 6-BA+0.4mg/L IBA时愈伤状态最好。
Experiment was carried out by using tender stem segments from Clematis‘Bill MacKenzie＇as explants to evaluate effect of sterilization time and types of basic culture medium on callus induction;based on the primary callus,effect on callus induction and their status under different combinations of exogenous hormones callus were also investigated.The results showed that the best sterilization time was 12 minutes.The basic medium was MS with 68.4%induction rate.All combinations of exogenous hormones could induced callus with different status,the best status was inducted with the medium of MS＋1.0mg/L 6-BA＋0.4mg/L IBA.
出处
《北方园艺》
CAS
北大核心
2015年第24期94-97,共4页
Northern Horticulture
基金
江苏省中国科学院植物研究所青年基金资助项目(SQ201301)
关键词
铁线莲
茎段
愈伤
诱导
Clematis
stem segments
callus
induction
