摘要
以毛梾茎段为外植体,研究了不同消毒方式、不同基本培养基类型、不同取材时间和不同激素组合对毛梾初代培养的影响。结果表明:0.1%氯化汞10min取得了比较理想的消毒效果,污染率和褐化率均较低;MS培养基为较适宜的初代培养基,其萌发时间、生长状况均优于其它培养基;外植体在MS+6-BA 0.5mg/L+NAA 0.1mg/L+IBA 0.1mg/L上生长最好。
Taking the stems of Cornus walteri Wanger.as explants,the disinfection methods,basic medium,different sampling time and different hormone affect the primary culture of Cornus walteri Wanger.were studied.The results showed that the best disinfection method was using 0.1%HgCl2 for 10 minutes,it had lower contaminated rate and browning rate;MS medium was the best one for primary culture,the germination time and growth conditions were better than other medium;explants grew well in the medium of MS＋6-BA 0.5 mg/L＋NAA 0.1 mg/L＋IBA 0.1 mg/L.
出处
《北方园艺》
CAS
北大核心
2012年第7期123-125,共3页
Northern Horticulture
基金
林业公益性行业专项资助项目(200804010)
关键词
毛梾
初代培养
影响因素
Cornus walteri Wanger.
initial culture
influencing factors
