摘要
以沙田柚自交和异交花柱为试材,采用高通量测序技术对其进行转录组测序。通过差异分析得到RNase-like贮藏蛋白的基因序列,并研究了其理化性质。结果表明:该基因全长1 048bp(GenBank登录号为KR363153),开放阅读框(ORF)全长为693bp,共编码230个氨基酸,编码的蛋白质的分子量为25.82kDa,理论等电点为5.30,含有核苷酸结合的保守区域,属于RNase-T2超家族成员。该蛋白为疏水性蛋白,可能的磷酸化位点共有15个。氨基酸序列同源性分析表明,该基因编码的氨基酸与甜橙和克莱门柚的同源性均为99%。系统进化树显示沙田柚RNase-like贮藏蛋白基因与甜橙,克莱门柚的亲缘关系较近,属于同一进化分支。
The self-pollinated and cross-pollinated style of Citrus grandis var.Shatinyu Hort were used as test materials and transcriptome sequenced by high-throughput sequencing technology.The sequence of RNase-likestorage protein was obtained by differential analysis,what＇s more,the physical and chemical properties of this protein were studied.The result showed that,the length of this gene（GenBank accession number：KP172529）was 1 048 bp with an open reading frame（ORF）of 693 bp,encoding 290 amino acids with deduced molecular weight of 25.82 kDa,and the theoretical pI value was 5.30.And it belonged to the RNase-T2 superfamily with a conserved region binding the nucleotide.This protein was a hydrophobic protein,and there were 15 phosphorylation sites within the polypeptide chain.The homology analysis of amino acid sequence indicated that the RNase-like storage protein shared high homology with Citrus sinensis（99%）and Citrus clementina（99%）.The phylogenetic tree revealed that RNase-like gene storage protein showed closer kinship with Citrus sinensis and Citrus clementina,indicating that they belonged to the same evolutionary branch.
出处
《北方园艺》
CAS
北大核心
2015年第24期70-74,共5页
Northern Horticulture
基金
国家自然科学基金资助项目(31360477)
广西教育厅资助项目(2013YB036)
