摘要
研究发现,莲藕根状茎干物质、可溶性糖及可溶性蛋白含量随着根状茎发育而逐步增加,尤其在根状茎发育后期含量增加更加明显。在此基础之上,利用转录组测序技术,归纳并分析86个可能与莲藕根状茎膨大相关基因,分别为35个激素诱导蛋白基因、4个光诱导蛋白(MADS-BOX)基因、11个根状茎贮藏蛋白基因(Patatin)、35个与淀粉代谢相关基因以及1个与根状茎形成相关基因。数字基因差异表达谱研究结果表明,根状茎贮藏蛋白家族基因(Lrplp6、Lrplp9、Lrpfp1、Lrprp、Lrpfp2、Lrplp4、Lrplp5、Lrplp8、Lrpp、Lrplp2)和淀粉合成相关基因(Lrgbss、Lrsbe1、Lrsbe2、LrsbeII、LrsbeIII)在根状茎形成后期表达量高,表达丰度均为初期的3倍以上,而其他基因变化相对较小。半定量RT-PCR结果进一步证明上述数字基因差异表达谱结果的可靠性,同时也表明Lrplp8和Lrgbss的表达与根状茎膨大具有高度相关性。由此认为,上述10个贮藏蛋白合成相关基因和5个淀粉合成相关基因,尤其Lrplp8和Lrgbss,对莲藕根状茎的膨大起到重要作用。
It was found that the contents of dry matter,soluble sugar and soluble protein were enhanced with the growth and development of lotus（Nelumbo nucifera Gaertn）rhizome,especially in the later enlarging period of rhizome. In present study,transcriptome sequencing technologies were used to analysis the genes involved in development of lotus rhizome included 35 hormone related protein genes,4 light induced protein genes,35 starch metabolisms related genes and 1 tuberization gene,which might be related to enlarging of lotus rhizome were found. The result of digital gene expression profiles showed that ten Patatin family genes（Lrplp6,Lrplp9,Lrpfp1,Lrprp,Lrpfp2,Lrplp4,Lrplp5,Lrplp8,Lrpp, Lrplp2）and five starch metabolism related genes（Lrgbss,Lrsbe1,Lrsbe2,LrsbeII,LrsbeIII）significantly changed their expression levels with at least 3 fold in the later period compared with the initial period, while other genes did not obviously alter their expression. The result of Lrplp8 and Lrgbss temporal and spatial expression by semi-RT-PCR in lotus root was similar to the results revealed by digital gene expression profile,suggesting that these two genes were highly correlated with rhizome enlarging. Therefore,we speculated that above 10 Patatin genes and 5 starch metabolism genes played important roles in rhizome enlarging of lotus root.
出处
《园艺学报》
CAS
CSCD
北大核心
2012年第3期501-508,共8页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31071795)
国家公益性行业专项(200903017-02)
关键词
莲藕
根状茎
基因表达
激素
调控
lotus root
rhizome
gene expression
hormone
regulation
