摘要
目的检测ADAMTS4、Versican、Aggrecan在心肌梗死大鼠心脏中的表达。方法选择雄性Wistar大鼠构建心梗模型，随机分配15只人心梗组，5只入假手术组。手术后3d处死大鼠，取左心室制作冰冻切片，再采用免疫组织化学法检测ADAMTS4、Versican及Aggrecan蛋白表达。结果ADAMTS4在梗死边缘区域心肌细胞中有表达；在梗死周围区域毛细血管内皮细胞中ADAMTS4有弱表达，Versican呈强表达；Aggrecan在心梗组及假手术组的心肌组织及血管内膜均未见表达。结论ADAMTS4参与心梗后的炎症反应，且心梗后的血管中存在着ADAMTS4对Versican的降解；Aggrecan则未参与心梗的病理生理过程。
Objective To detect the expression of a disintegrin and metalloproteinase with thrombospondin motifs 4 （ADAMTS4）, Versican, and Aggrecan in hearts of myocardial infarction rats. Methods Male Wistar rats were chosen to make myocardial infarction model. Fifteen rats were randomly assigned into the myocardial infarction group and five rats into the sham-operation group. Rats were killed 3 d after surgery and the left ventricles were taken to produce frozen section. ADAMTS4, Versican, and Aggrecan protein expressions were detected by immunohistochemistry method. Results ADAMTS4 expressed in myocardial cells at marginal zone of the infarcted area. ADAMTS4 expressed weakly while Versican expressed strongly in capillary endothelial cells of marginal zone of the infarcted area. Aggrecan showed no expression in the cardiac tissue and vascular endothelium of both myocardial infarction group and sham-operation group. Conclusions ADAMTS4 is involved in the inflammation after myocardial infarction. Degradation of ADAMTS4 to Versican exists in the vessels after myocardial infarction. Aggrecan is not involved in the pathophysiological process of myocardial infarction.
出处
《国际生物医学工程杂志》
CAS
2015年第5期286-289,I0007,共5页
International Journal of Biomedical Engineering
基金
山东省潍坊市卫生局科研基金项目（2013025）
