摘要
以柞蚕害虫黑广肩步甲(Calosoma maximociczi Morawitz)的成虫为材料,分离其肠道细菌并从中筛选产脂肪酶的菌株,了解害虫肠道细菌菌群结构,寻找具有应用价值的高产脂肪酶的微生物资源。从黑广肩步甲成虫的肠道中共分离出21个好氧细菌分离株,进一步利用选择性培养基筛选产脂肪酶的菌株并检测酶活力,获得4株产脂肪酶的优势菌株。经过生理生化特性测试和16S r DNA序列分析,确定4株产脂肪酶的菌株分别属于变形杆菌属(Proteus sp.)、沙雷氏菌属(Serratia sp.)和芽孢杆菌属(Bacillus sp.)。研究结果表明,黑广肩步甲成虫肠道内的细菌种类丰富,分离的4株产脂肪酶菌株的产酶活力较高,可进一步研究评价其利用价值。
The adults of Calosoma maximociczi Morawitz were used as material for separating intestinal bacteria and li- pase-producing microbial strains to ascertain bacterial composition in intestine of C. maximociczi and find microbial re- sources with high yield of lipases and promising utilization value. Totally 21 strains of aerobic bacteria were isolated from intestinal canal of C. maximociczi adults. Further 4 strains of dominant lipase-producing bacteria were obtained through selective media and determination of lipase activity. Based on physiological and biochemical characteristics and 16S rDNA sequence analysis result, these lipase-producing bacteria were identified to belong to Proteus sp., Serratia sp. and Bacillus sp., respectively. The results indicated that there were abundant bacteria in the intestinal canal of C. maximociczi adults. The isolated lipase-producing strains had high enzymatic activity for further evaluation on their utilization value.
出处
《蚕业科学》
CAS
CSCD
北大核心
2015年第6期1044-1049,共6页
ACTA SERICOLOGICA SINICA
基金
现代农业产业技术体系建设专项(No.CARS-22)
国家茧丝绸发展专项资金项目
辽宁省自然科学基金项目(No.2014-027002)
山东省自然科学基金项目(No.ZR2012CQ008)
