摘要
目的 探讨青藤碱对大鼠肾缺血再灌注时NF-KB及诱导型一氧化氮合酶（iNOS）水平的影响.方法 健康成年雄性Wistar大鼠72只,6～9周龄,体重180～220 g,采用随机数字表法分4组（n=18）：对照组（C组）、青藤碱组（SIN组）、肾缺血再灌注组（I/R组）和青藤碱＋肾缺血再灌注组（SIN＋I/R组）.采用夹闭左肾肾蒂45 min,再灌注即刻切除右肾的方法制备肾缺血再灌注损伤模型.SIN＋I/R组再灌注前30 min、SIN组于相应时点腹腔注射青藤碱60 mg/kg.于再灌注6、8和12h时,心脏穿刺采集血标本,测定血清Cr和BUN浓度.取左肾组织,光镜下观察肾脏病理学结果,采用非生物素二步免疫组织化学法检测大鼠肾脏NF-KB阳性细胞率及iNOS的表达.结果 与C组比较,I/R组和SIN＋I/R组血清Cr和BUN浓度升高,肾组织NF-KB阳性细胞率升高,iNOS表达上调（P＜0.05）,SIN组上述指标差异无统计学意义（P＞0.05）;与I/R组比较,SIN＋I/R组血清Cr和BUN浓度降低,肾组织NF-κB阳性细胞率降低,iNOS表达下调（P＜0.05）.SIN＋I/R组肾组织病理学损伤较I/R组减轻.结论 青藤碱减轻大鼠肾缺血再灌注损伤的机制与抑制NF-KB活性,下调iNOS表达有关.
Objective To investigate the effect of sinomenine on the levels of nuclear factor kappa B （NF-κB） and inducible nitric oxide synthase （iNOS） during renal ischemia-reperfusion （I/R） in rats.Methods Seventy-two healthy male Wistar rats, aged 6-9 weeks, weighing 180-220 g, were randomly assigned into 4 groups （n =18 each） using a random number table： control group （group C）, sinomenine group （group SIN）, group I/R, and sinomenine＋I/R group （group SIN＋I/R）.The animals were anesthetized with 10% chloral hydrate 350 mg/kg.The left renal pedicles were clamped with atraumatic microclips for 45 min followed by reperfusion, and the right kidney was removed immediately after onset of reperfusion to establish the model of renal I/R injury.Sinomenine 60 mg/kg was injected intraperitoneally at 30 min before reperfusion in group SIN ＋I/R, and at the corresponding time point in group SIN.At 6, 8 and 12 h of reperfusion, the blood samples were drawn by cardiac puncture for measurement of serum creatinine （Cr） and blood urea nitrogen （BUN） concentrations.The left renal specimens were obtained for examination of pathological changes （with light microscopes） and for determination of the rate of NF-κB-positive cells and iNOS expression in renal tissues （by immunohistochemistry）.Results Compared with group C, the concentrations of serum Cr and BUN, and rate of NF-κB-positive cells were significantly increased, and the expression of iNOS was up-regulated in I/R and SIN＋I/R groups, and no significant change was found in the parameters mentioned above in group SIN.Compared with group I/R, the concentrations of serum Cr and BUN, and rate of NF-κB-positive cells were significantly decreased, and the expression of iNOS was down-regulated in group SIN＋I/R.The microscopic examination showed that the pathological changes of kidney were significantly attenuated in group SIN＋I/R compared with group I/R.Conclusion The mechanism by which sinomenine attenuates renal I/R injury is related to inhibited activity of NF-κB and down-regulated expression of iNOS in rats.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2015年第8期951-954,共4页
Chinese Journal of Anesthesiology
