摘要
目的 探讨急性胰腺炎时核因子κB (NF κB)激活的水平 ;及其肿瘤坏死因α(TNF α)在胰腺炎发病中的作用。方法 将 6 4只Wistar大鼠分成对照组和胰腺炎两组 ,在复模后分别于1 5、3、6、12h用流式细胞术 (FCM )和酶联免疫吸附法 (ELISA)分别检测了NF κB激活的水平和TNF α蛋白表达量。同时测定血清中淀粉酶及脂肪酶含量 ,并行病理切片进行胰腺病理分级。结果 急性胰腺炎组中的NF κB激活的水平和TNF α蛋白表达量在各个时间段均有所增高 (P <0 0 5 )。在复模后 3h胰腺组织中NF κB激活的水平达到高峰 ,TNF α蛋白表达量和血清中淀粉酶及脂肪酶升高最明显 (P <0 0 1) ;胰腺组织充血、水肿明显 ,至 6hNF κB激活趋缓 ,而TNF α蛋白表达量和血清中淀粉酶及脂肪酶仍处于升高趋势 ,同时胰腺组织出现大片的出血及坏死。结论 在急性胰腺炎的发病过程中 ,NF κB的活化作为始动因子激活一系列的炎症介质 ,尤以TNF α最为明显 ,同时大量的消化酶类物质释放 ,引起胰腺组织快速的炎症反应 。
Objective To explore the effect of the activated NF κB and the interaction between activated NF κB and tumor necrosis factor (TNF) α in the process of acute pancreatitis. Methods 64 Wistar rats were randomly divided into two groups of 32 rats: pancreatitis group (to be made pancreatitis models) and control group. 1.5, 3.0, 6.0, and 12.0 hours after the onset of experiment the amount of ascitic fluid was measured, blood was extracted from abdominal aorta, changes of pancreas was observed, pancreatic tissues were stained with HE, and flow cytometry (FCM) and enzyme linked immuno sorbent assay (ELISA) were used to examine the content of TNF α protein and activation of NF κB (number of positive cells/50 μl) in the pancreatic tissues. The contents of amylase and lipase in plasma were examined. The pathology of pancreatic tissue was graded. Results The levels of activated NF κB and TNF α protein in the pancreatic tissue were all significantly higher than those of the control group at any rime point (all P <0.05). The levels of activation of NF κB of the pancreatitis group at any time point were significantly higher than those of the control group (all P <0.01) and reached its maximum about 3.0 hours after the onset of experiment and then declined. The levels of plasma amylase and lipase were significantly higher in the pancreatic group than in the control group at any time point. (all P <0.01). In the pancreatic group severe edema and congestion were found at the 3.0 h time point and bleeding and necrosis were found at the 6.0 hour time point. Conclusion Activated NF κB as initial factor plays an important role in the pathogenesis of acute pancreatitis, activates a lot of inflammatory media, and induces cascading reaction of inflammation . TNF α is a pivotal factor in pancreatitis pathogenesis, it cooperates with amylase and lipase to intensify the leisure in pancreatic tissue resulting bleeding and necrosis.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2003年第17期1497-1500,共4页
National Medical Journal of China
