摘要
目的:考察糖槭果中多糖的最佳闪式提取工艺。方法:利用闪式提取法提取多糖,苯酚硫酸法进行含量测定,以糖槭果多糖的得率为指标,以提取时间、提取电压、料液比为主要考察因素,正交试验法筛选闪式提取糖槭果多糖的最佳工艺,与传统煎煮法比较多糖的提取率。结果:当提取电机电压225 V,料液比1:25,提取时间90 s,糖槭果多糖的提取率为19.92%,且提取率高于传统煎煮法的17.99%。结论:闪式提取法可以明显提高糖槭果多糖的提取率,该方法方便、快捷和高效。
Objective：To optimize homogenate extracting process of Acer saccharum Marsh fruit polysaccharide（ASFP）. Methods：By taking the extraction ratio of ASFP as evaluation parameter,the total carbohydrate content was determined according to the phenol-sulphuric acid method,considering the the motor voltage,extraction time and solid-liquid ratio as affecting factors,the homogenate extracting process of ASFP was optimized by orthogonal design. The homogenate extracting process was compared with the method of traditional decocting extraction. Results：The optimum condition was achieved when extraction voltage was 225 V,solid to liquid ratio（g/m L）was 1∶25 and extraction time was 90 s,the extraction rate of total ASFP is 19.92% and higher than the traditional decocting method. Conclusion：The homogenate extraction was feasible and reliable for extracting polysaccharide from Acer saccharum Marsh fruit. It is a better and more efficient method than traditional decocting extraction.
出处
《辽宁中医药大学学报》
CAS
2015年第10期42-44,共3页
Journal of Liaoning University of Traditional Chinese Medicine
基金
黑龙江省教育厅科学研究面上项目(12541818)
关键词
糖槭果
多糖
闪式提取
正交试验
Acer saccharum Marsh fruit
polysaccharide
homogenate extraction
orthogonal test
