摘要
目的:研究糖槭叶的抗氧化活性,为开发新的药用资源提供理论基础和科学依据。方法:通过ESR测定DPPH抑制率、SOD Assay Kit-WST法测定SOD活性、Griess法测定NO的消去作用对糖槭叶多糖体外抗氧化活性进行考察;通过测定小鼠血清中SOD活性和MDA含量对糖槭叶进行体内抗氧化活性研究。结果:测定糖槭叶多糖对DPPH的抑制率为(12.2±6.74)%,SOD活性为(0.27±0.003)U/mg,对NO的消去率为(53.0±2.1)%;空白组、模型组、小剂量组、大剂量组的小鼠血清中SOD活性分别为(16.20±3.00)U/ml、(9.72±0.69)U/ml、(39.70±8.57)U/ml、(22.56±3.08)U/ml,MDA含量分别为(27.43±2.28)nmol/ml、(35.96±5.08)nmol/ml、(15.29±4.02)nmol/ml、(17.70±8.14)nmol/ml。结论:糖槭叶具有显著的抗氧化活性。
Objective：To study the antioxidant activity of leaves of Acer Saccharum,which will afford the scientific foundation and academic basis for eveloping the new pharmic resources.Methods： Samples of ethanol extraction were studied on pharmacological activities through measuring restraining ratio to DPPH,measuring SOD activities with Assay Kit-WST and measuring the reduction to NO with Griess for antioxygenation outside,and measuring activity of SOD and the quantity of MDA for antioxygenation in body.Results：The restrain ratio to DPPH was（12.2±6.74）%,activity of SOD was（0.27±0.003）U/mg,the ratio of reduction to NO was（53.0±2.1）%;the activities of SOD in blank group,model group,low-dose group and high-dose group were（16.20±3.00）U/ml,（9.72±0.69）U/ml,（39.70±8.57）U/ml,（22.56±3.08）U/ml,and the quantity of MDA was（27.43±2.28） nmol/ml,（35.96±5.08）nmol/ml,（15.29±4.02）nmol/ml,（17.70±8.14）nmol/ml.Conclusion：Leaves of Acer Saccharum have a high antioxidant activity.
出处
《中医药信息》
2011年第4期10-11,共2页
Information on Traditional Chinese Medicine
基金
佳木斯大学科学研究项目面上项目(S2010-60)
黑龙江省自然科学基金面上项目(201064)
黑龙江省教育厅科学技术研究项目面上项目(11551507)
关键词
糖槭叶
抗氧化
SOD
MDA
Leaves of acer saccharum
Antioxidant
SOD
MDA
