摘要
目的:在10种清热解毒药中筛选出抗氧化活性较高的药物,为筛选高效治疗糖尿病的中药和新药提供一定的理论基础。方法:测定清热解毒药醇提液对DPPH·的清除率,并计算半数清除率(IC50),以维生素E为阳性对照,评价其抗氧化活性。结果:不同中药的醇提液在一定浓度范围内,均有较强的体外抗氧化活性,其中连翘的最大效能及强度均最强,且IC50值是0.312 7 g·L-1为最小。结论:连翘抗氧化活性最强。本研究为连翘治疗糖尿病的作用机制以及应用前景提供了新思路,也为临床合理应用提供了参考。
Objective：To give some new advises and insights to the research of new traditional Chinese medicine,we measured the antiox-idant activity in vitro of ten samples of heating-clearing and detoxicating drugs. Methods：Use microplate method to determine the antiox-idant activity by cleaning DPPH· and calculate the half inhibition concentration（IC50 ）. If the value of IC50 was smaller,it indicated the antioxidant activity was stronger. Results：Different traditional Chinese medicine has different antioxidant activity in vitro,the dose-efficiency curve shows that the potency and efficacy of forsythia was strongest and the IC50 of forsythia was 0. 3127 mg·mL - 1 that of smallest. Conclusion：The antioxidant activity of forsythia was strongest. It provides a new way to research the mechanism of action of for-sythia for treating diabetes and application prospect.
出处
《中医学报》
CAS
2015年第9期1329-1331,共3页
Acta Chinese Medicine
基金
国家自然科学基金面上项目(81473435)
河南省教育厅科学技术重点资助项目(14A310023)
河南中医学院大学生创新学习项目{CXXM[2014]14}
河南省科技厅科技攻关重点项目(144300510015)
河南中医学院博士科研基金项目(BSJJ2009-37)
关键词
清热解毒药
连翘
DPPH·法
抗氧化活性
heating-clearing and detoxicating drugs
forsythia
DPPH· method
antioxidant activity
