摘要
目的:观察喘可治注射液对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)大鼠血清C反应蛋白(C-reactive protein CRP)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)、白细胞介素-8(interleukin-8,IL-8)及肺功能的影响。方法:36只SD大鼠随机平均分为对照组、模型组及喘可治组,采用烟熏和内毒素注射的方式建立COPD模型。喘可治组给予喘可治3.75 mg·(kg·d)-1腹腔注射进行治疗,其余两组给予等量生理盐水。检测3组大鼠的血清CRP、TNF-α、IL-6、IL-8及肺功能。结果:1血清炎症因子:模型组大鼠血清中CRP、TNF-α、IL-6、IL-8含量高于对照组[(4.23±0.62)mg·L-1vs(0.94±0.11)mg·L-1,(181.16±22.18)ng·L-1vs(54.32±7.07)ng·L-1,(171.84±22.84)ng·L-1vs(44.13±6.61)ng·L-1,(156.84±21.84)ng·L-1vs(54.13±10.61)ng·L-1](P<0.05);喘可治组大鼠血清中CRP、TNF-α、IL-6、IL-8含量低于模型组[(1.85±0.24)mg·L-1vs(4.23±0.62)mg·L-1,(89.45±10.23)ng·L-1vs(181.16±22.18)ng·L-1,(76.62±8.94)ng·L-1vs(171.84±22.84)ng·L-1,(81.34±9.37)ng·L-1vs(156.84±21.84)ng·L-1](P<0.05);2肺功能:模型组大鼠的跨肺压、气道流速高于对照组[(5.24±0.70)vs(4.02±0.65)cm H2O,(0.314±0.042)m L·s-1vs(0.239±0.035)m L·s-1](P<0.05),潮气量低于对照组(0.481±0.055)m L vs(0.625±0.082)m L(P<0.05);喘可治组大鼠跨肺压、气道流速低于对照组[(4.51±0.61)cm H2O vs(5.24±0.70)cm H2O,(0.252±0.037)m L·s-1vs(0.314±0.042)m L·s-1](P<0.05),潮气量高于对照组(0.569±0.072)vs(0.481±0.055)m L(P<0.05)。结论:喘可治注射液治疗可以缓解COPD大鼠的炎症反应、改善肺功能。
Objective：To study the effect of Chuankezhi on serum CRP,TNF-α,IL-6,IL-8 and lung function of rats with chronic obstruc-tive pulmonary disease. Methods：Adult male SD rats were divided into control group,model group and Chuankezhi group. Smoking and endotoxin injection way were used to build COPD model and Chuankezhi were given by intraperitoneal injection for therapy. Then detect serum CRP,TNF-α,IL-6,IL-8 and lung function index. Results：①Serum inflammatory cytokines：serum CRP,TNF-α,IL-6,IL-8 con-tents of model group were higher than those of control group[（4. 23 ± 0. 62）mg·L - 1 vs（0. 94 ± 0. 11）mg·L - 1 ,（181. 16 ± 22. 18）ng·L - 1 vs（54. 32 ± 7. 07）ng·L - 1 ,（171. 84 ± 22. 84）ng·L - 1 vs（44. 13 ± 6. 61）ng·L - 1 ,（156. 84 ± 21. 84）ng·L - 1 vs 54. 13 ± 10. 61）ng·L - 1 ]（P ﹤ 0. 05）;serum CRP,TNF-α,IL-6,IL-8 contents of chuankezhi group were lower than those of model group[（1. 85 ± 0. 24）mg·L - 1 vs（4. 23 ± 0. 62）mg·L - 1 ,（89. 45 ± 10. 23）ng·L - 1 vs（181. 16 ± 22. 18）ng·L - 1 ,（76. 62 ± 8. 94）ng·L - 1 vs（171. 84 ± 22. 84）ng·L - 1 ,（81. 34 ± 9. 37）ng·L - 1 vs（156. 84 ± 21. 84）ng·L - 1 ]（P ﹤ 0. 05）. ②Pulmonary function：transpulmonary pressure and airway flow rate of model group were higher than those of control group[（5. 24 ± 0. 70）vs （4. 02 ± 0. 65）cmH2 O,（0. 314 ± 0. 042）mL·s - 1 vs（0. 239 ± 0. 035）mL·s - 1 ]（P ﹤ 0. 05）,tidal volume was lower than that of control group（0. 481 ± 0. 055）mL vs（0. 625 ± 0. 082）mL（P ﹤ 0. 05）;transpulmonary pressure and airway flow rate of Chuankezhi group were lower than those of model group[（4. 51 ± 0. 61）cmH2 O vs（5. 24 ± 0. 70）cmH2 O,（0. 252 ± 0. 037）mL·s - 1 vs （0. 314 ± 0. 042）mL·s - 1 ]（P ﹤ 0. 05）,tidal volume was higher than that of model group（0. 569 ± 0. 072）vs（0. 481 ± 0. 055）mL （P ﹤ 0. 05）. Conclusion：Chuankezhi injection therapy can relieve inflammatory reaction and improve pulmonary function of COPD rats.
出处
《中医学报》
CAS
2015年第9期1256-1258,共3页
Acta Chinese Medicine
基金
湖北省科技攻关项目(20140620331)
