摘要
目的：研究草豆蔻抑制实验性脓毒症的有效成分,探讨其作用机制。方法：采用盲肠结扎穿孔和内毒素注射造成小鼠脓毒症模型,记录小鼠存活率。采用试剂盒检测小鼠巨噬细胞培养上清中NO、PGE2含量以及iNOS活力。结果：草豆蔻各提取部位中,乙酸乙酯相抗脓毒症作用最强,正丁醇相次之,石油醚和水相无明显作用;乙酸乙酯相中,豆蔻明的作用最强,反,反-1,7-二苯基-4,6-庚二烯-3-酮和山姜素次之,乔松素无明显作用。豆蔻明（6.25-50μM）浓度依赖性抑制LPS诱导小鼠腹腔巨噬细胞产生NO、PGE2以及iNOS活力。此外,豆蔻明（10,30,100mg/kg）局部用药显著抑制巴豆油诱导的小鼠耳廓肿胀。结论：豆蔻明是草豆蔻抗脓毒症的主要有效成分。
Objective： To screen for the active constituents of Alpinia katsumadai Hayata against experimental sepsis and study the mechanisms of action. Methods： Experimental sepsis was induced by cecal ligation and puncture （CLP） or endotoxin injection in mice, and survival of septic mice was counted every 12 h for 96 h and 48 h, respectively. The content of NO and PGE2 and the activity of iNOS in the supernatants of mouse peritoneal macrophages were determined by commercial kits. Results： The acetic ether fraction of A. katsumadai Hayata showed more significant inhibitory activity against septic mouse death than other fractions. Cardamonin isolated from this fraction significantly elevated survival of septic mice and inhibited production of NO and PGE2 from primary mouse macrophages, Additionally, cardamonin remarkably inhibited mouse ear edema induced by croton oil. Condusion： Cardamonin is the main active constituents of A. katsumadai against sepsis.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2008年第3期54-57,共4页
Pharmacology and Clinics of Chinese Materia Medica
关键词
草豆蔻
豆蔻明
脓毒症
Alpinia katsumadai
cardamonin
sepsis
anti-inflammation
