摘要
以粉葛幼嫩茎尖和腋芽作为外植体,研究了不同浓度6-BA对无菌体系构建的影响,以及不同浓度6-BA,IAA,KT对丛生芽增殖的影响。根据丛生芽的生长特征筛选最佳生长培养基,并依据正交实验,以生长率为指标,优选适合丛生芽增殖的最佳培养条件。结果 MS+6-BA 2 mg/L+IAA 0.2 mg/L为最适启动培养基,MS+6-BA 0.1 mg/L+KT 0.1 mg/L+IAA 0.05 mg/L为最适增殖培养基。
Here we use shoot tip and axillary bud of Pueraria thomsonii Benth as explant, the effects of different concentrations of 6 - BA effects on sterile system building and different concentrations of 6-BA, IAA, KT on multiple shoot clumps proliferation are studied. According to the growth characteristics of multiple shoot clumps to screen the best growth medium, and on the basis of orthogonal experiment, the growth rate as index, to select the best culture conditions for multiple shoot clumps proliferation. Results show that the MS＋6-BA 2 mg/L＋ IAA 0.2 mg/L is the best initial medium, MS ＋ 6-BA 0.1 mg/L ＋ KT 0.1 mg/L ＋ IAA 0.05 mg/L is the best Enrichment Medium.
出处
《辽宁农业职业技术学院学报》
2015年第4期12-13,23,共3页
Journal of Liaoning Agricultural Technical College
关键词
粉葛
茎尖
腋芽
组织培养
Pueraria Thomsonii Benth
shoot tip
axillary bud
tissue culture
