摘要
对野葛高频植株再生体系相关因子进行优化,结果表明,野葛带芽茎段再生体系的最佳消毒方式为70%酒精处理30s后用0.1%HgCl2处理15min;最佳培养基为MS+NAA 0.5mg/L+6-BA 5.0mg/L;最佳培养条件为:蔗糖浓度30g/L,pH 6.0,液体培养,培养温度25℃,光照培养。
The effects of several factors on dedifferentiation and redifferentiation of Pueraria lobata earthnut were studied. The results showed that the best disinfection way of Pueraria lobata stems with a bud was firstly treated with 70% ethyl alcohol for 30 seconds then treated with 0.1% HgCl2 for 15 minutes, and the optimum regeneration culture medium was MS＋NAA 0.5mg/L ＋ 6-BA 5.0mg/L. Liquid culture with sucrose 30g/L, pH 6.0, cultured in light at 25℃ gave a best result for high frequency plant regeneration ofPueraria lobata.
出处
《亚热带植物科学》
2008年第1期29-32,共4页
Subtropical Plant Science
基金
江西省自然(青年)科学基金(0630103)
江西上饶师范学院2008-2009年度院级科技项目
关键词
野葛
高频植株再生体系
带芽茎段
组织培养
Pueraria lobata
high frequency plant regeneration
stem with a bud
tissue culture
