摘要
奶牛乳腺上皮细胞不仅具有合成和分泌乳汁的功能,而且在乳腺的先天免疫中扮演着重要角色,对泌乳机制、乳房炎发病机制的研究,以及药物筛选具有重要意义。原代培养的奶牛乳腺上皮细胞适宜建立细胞模型,可作为生理、病理、药理等方面研究的良好介质,解决体内试验周期长、成本高、个体差异大的难题。作者主要从奶牛乳腺上皮细胞原代培养的发展历程、培养技术、纯化技术及鉴定方法等方面的最新研究情况进行综述,以期为奶牛乳腺上皮细胞培养相关研究提供参考。
The bovine mammary epithelial cells not only have the function of synthesis and secre- tion of milk, but also play an important role in innate immunity system of the mammary gland,and there is great significance of them on studying the mechanism of lactation,mastitis pathogenesis and drug screening. Primary cultured bovine mammary gland epithelial cells are suitable for set- ting up cell model which can be used as the dielectric for physiological, pathological and pharma- cological researching, avoiding the difficulties of in vivo test, such as the long cycle, the high cost, the individual difference, etc. The author summarized the latest researches of cell primary culture in vitro, cultivation technology, purification and identification method in order to provide reference for the studies of bovine mammary epithelial cells culture.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第8期2109-2115,共7页
China Animal Husbandry & Veterinary Medicine
基金
国家奶牛产业技术体系(CARS37)
关键词
奶牛
乳腺上皮细胞
原代培养
鉴定
bovine
mammary epithelial cells
primary culture
identification
