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荷斯坦奶牛乳腺上皮细胞的体外培养 被引量:14

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摘要 用外科手术的方法采取健康的泌乳期荷斯坦奶牛乳腺组织,采用机械剪切结合酶消化的方法,在体外进行原代乳腺上皮细胞分离培养。结果成功地培养出荷斯坦奶牛原代乳腺上皮细胞。显微镜下观察,细胞形成单层呈鹅卵石样;角蛋白免疫组化染色后,呈现阳性反应。经数次传代后,细胞增殖依旧旺盛。
出处 《畜牧与兽医》 北大核心 2007年第4期41-43,共3页 Animal Husbandry & Veterinary Medicine
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参考文献16

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二级参考文献27

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