摘要
为促进牛耳秋海棠的快繁及工厂化生产,以牛耳秋海棠的叶片为外植体,对其离体再生培养技术进行了研究。结果表明：牛耳秋海棠最佳消毒方法为75%酒精消毒8s,0.1%的HgCl2消毒6~7min（滴加一滴吐温）;牛耳秋海棠愈伤组织诱导和分化的最佳培养基为MS＋6-BA0.5~1.0mg·L^-1＋NAA0.1mg·L^-1,诱导率达85.42%~87.08%;牛耳秋海棠不定芽增殖的最适宜培养基是MS＋6-BA1.0mg·L^-1＋NAA0.1mg·L^-1,增殖倍数高达42.43。
In order to improve the rapid propagation and factory production of Begonia sanguinea Raddi, taking leaves of Begonia sanguinea Raddi as explants, the tissue culture of Begonia sanguinea Raddi was studied. The results showed that the optimum sterilization time of explants were 8 s by 75% alcohol and 6-7 min by 0.1% mercuric chloride with a drop of Tween; The optimized medium for callus induction and differentiation was MS＋6-BA 0. 5-1.0 mg·L^-1＋NAA 0.1 mg·L^-1 ,the induction rate was 85.42%-87.08% ; The optimal multiplication medium was MS＋6-BA 1.0 mg·L^-1＋NAA 0.1 mg·L^-1 ,the multiplication coefficient was as high as 42.43.
出处
《黑龙江农业科学》
2015年第8期14-18,共5页
Heilongjiang Agricultural Sciences
关键词
牛耳秋海棠
不定芽
愈伤组织
组培快繁
Begonia sanguinea Raddi
adventitious bud
callus
tissue culture
