摘要
以肾蕨匍匐茎茎尖为外植体,对离体再生培养技术进行了研究。结果表明:GGB的最佳诱导培养基为MS+BA0.7 mg.L-1,诱导率为95%;GGB增殖培养基为MS+BA0.7 mg.L-1,增殖系数为5.3;孢子体诱导培养基为MS+BA0.7 mg.L-1+NAA0.7 mg.L-1,诱导率可达到82%;适合诱导生根培养基为MS+NAA0.2 mg.L-1+0.1%Ac,生根率为100%。
Using the stem apex of the stolon of Nephrolepis auriculata as explants,the study on in vito culture was conducted.The results showed that the most appropriate induction culture medium of GGB was MS ＋BA0.7 mg·L-1,the ratio of induction was 95%.The most proper multiplication medium was MS＋BA0.7 mg·L-1,multiplication coefficient was to be 5.3;The medium for GGB to shoot was MS＋BA0.7 mg·L-1＋NAA0.7 mg·L-1,rate of inducement was 82%;The most appropriate roots-planting culture medium was MS ＋ NAA0.2 mg·L-1＋ 1.0% Ac,and the rooting rate was up to 100%.
出处
《黑龙江农业科学》
2009年第5期20-22,共3页
Heilongjiang Agricultural Sciences
关键词
肾蕨
离体培养
绿色小体(GGB)
Nephrolepis auriculata
in vitro culture
green globular bodies（GGB）
