摘要
本文采用免疫荧光组织化学双标方法结合激光共聚焦显微镜技术研究了伤害性受体P2X2阳性感觉纤维末梢在小鼠食管内的分布及与降钙素基因相关肽(CGRP)阳性纤维的关系。结果表明:在食管各水平均可见到P2X2阳性感觉纤维末梢。P2X2阳性纤维集中分布于食管的内、外肌层之间,部分或全部的覆盖在肌间神经节的表面。一些串珠样的纤维深入到肌间神经节内,相互缠绕在一起形成许多结构复杂、形状各异的神经节内板状末稍(IGLEs)。荧光双标显示许多CGRP阳性纤维围绕在P2X2阳性IGLEs周围,没有见到P2X2和CGRP在同一纤维的共存。结状神经节内可见许多中小型神经元表达P2X2,P2X2/CGRP双标神经元稀少,而背根神经节内可见较多的P2X2阳性细胞含有CGRP。由此可见,小鼠食管内部分感觉神经末梢表达伤害性受体P2X2,并形成IGLEs样末梢结构。
The distribution of P2X2 immunoreactive afferent endings and the relationship with calcitonin gene-related peptide (CGRP) immunoreactive fibers in the mouse esophagus was studied using double immunofluorescence method combined confocal laser scanning microscope technique. P2X2 immunoreactive endings were seen in sections from all levels of the mouse esophagus. The distribution of P2X2 immunoreactive structures concentrated in the area between outer and inner muscular layers, covered myenteric ganglia totally or partly. The dense clusters of fine varicose P2X2 immunoreactive terminals protruded into myenteric ganglia forming complexes of profuse laminar structures for intraganglionic laminar endings (IGLEs). Double immunofluorescence for P2X2 and CGRP revealed that numerous varicose fibers immunoreactive for CGRP closely surrounded P2X2 immunopositive IGLEs. However, P2X2 immunoreactive terminals never expressed CGRP immunoreactivity. In nodose ganglion, numerous neuronal cell bodies in small- and middle-sized displayed P2X2 immunoreactivity. P2X2/CGRP double-labeling neurons were rare. However, many P2X2 positive neurons in dorsal root ganglion contained CGRP immunoreactivity. These results demonstrated that some afferent endings in mouse esophagus expressed nociceptor P2X2, and formed IGLEs structures.
出处
《山东农业大学学报（自然科学版）》
CSCD
2015年第4期540-543,共4页
Journal of Shandong Agricultural University：Natural Science Edition
基金
国家自然科学基金资助项目(81371234)
山东省自然科学基金资助项目(ZR2013HM032)
山东省医药卫生科技发展计划项目(2011HW080)
