摘要
目的:探讨大鼠食道感受机械牵拉刺激的迷走传入神经末梢-神经节内板状末梢(IGLEs)的特异结构及其特性。方法:①用苯乙烯基染料FM1-43结合牵拉刺激显示大鼠食道IGLEs的特异结构,与P2X2免疫组织化学染色比较,证明IGLEs是迷走神经感受机械刺激的受体;②用不同的药物(TTX,Ca2+,Benzamil,Capsaicin,α,-βmeATP)阻断或激活FM1-43进入IGLEs,探讨IGLEs感受机械刺激的机制。结果:牵拉刺激后,FM1-43染色显示的IGLEs的数目明显高于未牵拉组(P<0.01);FM1-43染色显示的IGLEs形态与P2X2免疫组织化学染色完全一致。TTX,Ca2+不影响牵拉刺激激活的FM1-43显示的IGLEs数目;Benzamil则可阻断FM1-43进入IGLEs。单独应用Capsaicin无需牵拉刺激,也可促使FM1-43进入IGLEs。α,-βmeATP不能激活IGLEs,FM1-43无法显示其结构。结论:①IGLEs是大鼠食道迷走神经的机械感受器;②IGLEs对机械刺激的传导不需要神经递质和动作电位的介导,而是直接通过机械敏感性离子通道,这种通道属于上皮钠离子通道家族。
To investigate the structure and characteristics of mechanotransduction by intraganglionic laminar endings （IGLEs） as the mechanoreceptors in rat gut. Methods： ① IGLEs were displayed by styryl dye FM1-43 with stretch stimulation to the rat esophagus. P2X2 immunohistochemistry staining was used to confirm that the structure displayed by FM1-43 was that of IGLEs; ② Different drugs （TTX, Ca^2＋ , Benzamil, Capsaicin, and α,β- meATP）were used to block or stimulate stretch-evoked IGLEs staining by FM1-43 for further investigating the mechanism of mechanotransduction by IGLEs. Results： ① The amount of IGLEs displayed by stretch-evoked FM1-43 staining was much more than that without stretch stimulation （P 〈 0.01）. The structure of IGLEs displayed by FM1-43 was consistent with that by P2X2 ;② TIX, Ca^2＋ had no effect on stretch-evoked IGLEs staining, while benzamil could significantly block it. Caqsaicin could activate the entrance of FM1-43 into IGLEs without any stretch, while α,β- meATP, and the potent ATP anologue could not stimulate the staining at rest status. Conclusion： ① IGLEs are the mechanoreceptors of vagal afferent endings in the gut;② IGLEs transduce the mechanical stimulation in the gut through some mechanosensitive ion channels which are a type of sodium ion channels. Neurotransmitters or action potentials are not involved in this process.
出处
《山东大学学报（医学版）》
CAS
北大核心
2007年第6期586-589,608,共5页
Journal of Shandong University:Health Sciences
