摘要
目的研究ATP受体P2X2、P2X3在大鼠脊神经节感觉神经元的表达、形态特点及与神经肽降钙素基因相关肽(CGRP)、钙结合蛋白calretinin的共存。方法应用激光共聚焦显微镜结合免疫组织化学双标技术。结果免疫组织化学显示脊神经节内可见大量P2X2、P2X3受体阳性的细胞和神经纤维;P2X2受体亚型阳性细胞多为大、中、小型神经元,P2X3受体亚型阳性细胞为中、小型神经元;免疫组织化学双标显示有41.2±1.9%P2X2受体阳性细胞含有CGRP,而P2X3受体亚型与CGRP共存的细胞很难见到;有8.9±1.1%的P2X2免疫阳性神经元和7.2±1.3%P2X3受体阳性神经元分别含有calretinin。结论这一结果表明大鼠脊神经节内表达的ATP受体P2X2、P2X3是不同的受体亚型。
Objective： To study the expression of purinergic receptor, P2X2 and P2X3 subunites in sensory neurons of the rat dorsal root ganglion, their morphology and co - localization with neuropeptide, calcitonin gene - related peptide （CGRP） and calcium binding- protein, calretinin. Methods： -Laser scanning confocal microscope combined with immunohistochemical double labeling methods were used. Results： Most of P2X2 and P2X3 imrnunoreactive sensory neurons and fibers were observed in the dorsal root ganglion. The expression of P2X2 receptor was found in large-, middle- and small -sized cell bodies and P2X3 receptor in middle - and small - sized neurons. Immunohistoehemieal double - labeling showed that about 41.2 ± 1.9% P2X2 immunoreactive neurons co- stained with CGRP immunoreaetivity, but co- local- ization of P2X3 receptor with CGRP in sensory cell bodies was very few. There were 8. 9 ± 1.1% P2X2 immunopositive neurons and 7.2 ± 1. 3% P2X3 iremunoreaetive neurons contained ealretinin respeetivelyo Conclusion： These results demonstrated that P2X2 and P2X3 were different receptor subunites in sensory neurons of rat dorsal root ganglion.
出处
《泰山医学院学报》
CAS
2005年第4期288-291,共4页
Journal of Taishan Medical College
