摘要
目的:建立艾叶的UPLC指纹图谱鉴别方法,为其质量控制提供参考。方法:采用ZORBAX RRHD Eclipse Plus C18色谱柱(2.1 mm×100 mm,1.8μm),流动相乙腈-0.10%乙酸水梯度洗脱,流速0.20 m L·min-1,290 nm波长下测定艾叶及其混淆品指纹图谱,并作相似度比较分析。结果:建立了艾叶UPLC指纹图谱共有模式,有36个共有指纹峰被标定且相似度较高,而艾叶与非药用部位及混淆品的指纹图谱相似度均<0.700,共有峰的个数低于36个,具有明显的差异。结论:HPLC艾叶鉴别方法简便,快速,重复性好,特征性强。艾叶与非药用部位及混淆品的指纹图谱差异提示药材间的化学成分存在差异,相互不能混用。
Objective： To establish an UPLC method for fingerprints of Artemisae Argyi Folium,and provide reference for quality control of it. Method： The RP-UPLC method was achieved on a uitimate-C18（ 2. 1mm × 100 mm,1. 8 μm） column with mobile phase consisting of acetonitrile-0. 10% acetic acid water with gradient elution. Flow rate was set at 0. 20 m L·min- 1. The detection wavelength was set at 290 nm. Then established the fingerprints based on samples from different sources, and the similarity was analyzed. Result： The UPLC fingerprint was established with 36 common peaks. However similarity of the UPLC fingerprints of Artemisae Argyi Folium and non-medicinal parts and adulterants were lower than 0. 700 and the common peaks less than36. Conclusion： A simple,reproducible,and characteristic UPLC fingerprint was developed,which can be used to identify of Artemisae Argyi Folium. The difference of fingerprint indicated that of chemical compositions of Artemisae Argyi Folium,non medicinal parts and adulterants were varied,it should not be mixed.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第13期50-52,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
贵州省优秀科技教育人才省长专项(黔省专合字[2011]60号)
贵州省教育厅创新团队建设项目(黔教合人才团队[2013]12号)
