摘要
目的： 建立不同产地金银花药材的超高效液相特征性指纹图谱，为有效控制和科学评价金银花药材整体质量提供依据。 方法： 采用Agilent C18 色谱柱（2.1 mm×50 mm，1.8 μm），流动相乙腈-0.2%磷酸水，以0.4 mL·min-1的流速进行梯度洗脱，检测波长238 nm，柱温30 ℃。 结果： 在21 min内得到金银花药材的指纹图谱，对其中5个色谱峰进行了初步归属，并对14批药材样品进行了分析，其相似度为0.915-0.987。 结论： UPLC指纹图谱方法较HPLC大大缩短了分析时间，可用于金银花药材的质量评价。
Objective： To develop an UPLC method of determining the characteristic chromatographic profile of Lonicera japonica for controlling the drug quality quickly and accurately. Method： The analysis was performed on an Agilent C18 column（2.1 mm×50 mm,1.8 μm） with acetonitrile-0.2% phosphoric acid as mobile phase in gradient elution mode. The flow rate was 0.4 mL·min-1 and the injection volume was 2.0 μL with the detection wavelength of 238 nm.The column temperature was set at 30 ℃. Result： The fingerprint of Lonicera japonica was obtained within 21 min and five peaks in the fingerprint were designed.14 samples were analyzedand the similarities were 0.915-0.987. Conclusion： The UPLC system enjoys the merits of a higher speed,sensitivity,and resolution, it can be used to control the quality of L. japonica.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第2期67-69,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河南省教育厅自然科学研究计划项目(2010A360016)
