摘要
目的:优选疱疹康喷雾剂的提取工艺。方法:以盐酸小檗碱含量和出膏率的综合评分为指标,通过正交试验考察提取时间、提取次数、加水量对水提工艺的影响。以芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚总含量和出膏率的综合评分为指标,利用正交试验考察乙醇体积分数、提取时间、提取次数、乙醇用量对醇提工艺的影响。采用HPLC测定盐酸小檗碱及5个大黄苷元的含量。结果:最佳水提工艺为加12倍量水提取3次,每次75 min;盐酸小檗碱质量分数6.07%。最佳醇提工艺为加10倍量75%乙醇回流提取2次,每次75 min;5个大黄苷元总质量分数1.96%。结论:优选的提取工艺稳定可行,适用于疱疹康喷雾剂的工业化生产。
Objective： To optimize extraction process of Paozhenkang spray. Method： Taking composite score of the content of berberine hydrochloride and dry extract rate as index, orthogonal test was adopted to investigate influence of extraction time, extraction times, the amount of water on water extraction process. With composite score of the total content of aloe-emodin, rhein, emodin, chrysophanol, physcion and dry extract rate as index, orthogonal test was adopted to optimize alcohol extraction process by taking ethanol concentration, alcohol amount, extraction time and times as factors. Contents of berberine hydrochloride and five aglycones from Rhei Radix et Rhizoma were determined by HPLC. Result： Optimun water extraction process consisted of adding 12-fold of waters, decocting 3 times, 90 rain for each time, the content of berberine hydrochloride was 6.07%. Optimun alcohol extraction process was as following： refluxing extracted twice with 10-fold of 75% ethanol, 75 min for each time; the total content of five aglycones was 1.96%. Conclusion： These optimized extraction processes are stable and feasible for industrial production of Paozhenkang spray.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第10期30-33,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
疱疹康喷雾剂
盐酸小檗碱
芦荟大黄素
大黄苷元
综合评分
Paozhenkang spray
berberine hydrochloride
aloe-emodin
aglycones from Rhei Radix et Rhizoma
composite score
