摘要
目的对柿叶进行鉴别及对其总黄酮的提取工艺进行考察。方法通过显微鉴别、理化鉴别以及薄层色谱法对柿叶进行鉴别;通过单因素考察和正交设计试验,对柿叶中总黄酮进行提取工艺考察。结果柿叶的显微理化特征明显,薄层色谱法斑点显色清晰,柿叶中总黄酮的最佳提取工艺为70%乙醇提取2次,每次溶剂用量为10倍,每次提取时间为1.5h。结论柿叶的鉴别及其总黄酮提取工艺的研究为柿叶的开发利用提供了理论依据。
Objective To observe the identification of persimmon leaf and its extraction process of the total flavone.Methods The persimmon leaf was identified with microscope,physiochemical method and TLC.Through single factor and orthogonal design,the extraction process of the total flavone in persimmon leaf was observed.Results The microscopic and physiochemical characteristics of persimmon leaf were clear.TLC indicated distinct colored dots.The best extraction process：70% ethanol extraction for twice,10 times solvent,1.5 h for each extraction.Conclusion The study of the identification of persimmon leaf and its extraction process of the total flavone provides the theoretic evidence for the development and utilization of persimmon leaf.
出处
《世界中西医结合杂志》
2010年第6期500-501,共2页
World Journal of Integrated Traditional and Western Medicine
关键词
柿叶
鉴别
总黄酮
正交设计实验
Persimmon Leaf
Identification
Total Flavone
Orthogonal Design Test
